Computer-aided engineering of adipyl-CoA synthetase for enhancing adipic acid synthesisAdipic acidAdipyl-CoA synthetaseBiosynthesisEnzyme activityMolecular dynamics simulationThe combined computational-experimental approach provides an effective strategy for enhancing enzymatic characteristics, and the mutants may ...
In the future the malonyl-CoA and β-alanine routes towards 3HP could be combined in one strain. Here we describe the metabolic engineering of S. cerevisiae for high-level production of 3HP via the MCR pathway and characterize the production strain by 13C flux and transcription analysis....
form colorectal cancer organoids cost-effectively. These protocols can help assess biofunctional peptide hydrogel results on cellular adhesion and luminal development using immunostaining and fluorescence image evaluation. The mobile range used in this study is previously utilized for producing organoids in ...
Protein stain of BnDGAT1(1–116)His6 incubated with radiolabeled erucoyl-CoA is shown in lane 3. Full size image Additional evidence for the interaction of BnDGAT1(1–116)His6 with acyl-CoA was obtained using non-denaturing gel electrophoresis. When the fragment was preincubated with an ...
Full size image Figure 5 Analysis of E2F1, Sp1, KLF15 and E2F4 binding sites by site-directed mutagenesis and the predicted CpG island in the proximal ACSL1 promoter. (a) Site-directed mutagenesis was carried out in the construct pGL−325/+12. The different constructs were transiently trans...
I-peptide kinase <1> (<1> highly specifific for acetyl-CoA carboxylase, binds selectively to the dimeric form [11,14]) [11, 14] acetyl coenzyme A carboxylase kinase (phosphorylating) acetyl-CoA carboxylase bound kinase <1> [10] acetyl-CoA carboxylase kinase acetyl-CoA carboxylase kinase (cA...
(1989)). The eukaryotic form of ACCase is a homomeric, presumably cytosolic enzyme (also referred to as multi-functional (MF) enzyme) containing the BC, BCCP, and CT functional domains with a molecular weight of more than 200 kDa. The full-length homomeric ACCase has been cloned from ...