27-hydroxylanosterol (25R,26-hydroxylanosterol) was synthesized from lanosterol (Sigma-Aldrich, St. Louis, Missouri) by selective selenium dioxide oxidation using minor modifications of previous procedures (Camps et al., 1978). The final product has a mass spectrum identical to that of the natur...
HAFC was screened using a commercially available enzyme assay kit from Sigma-Aldrich Co. (St Louis, MO, USA). Nicotinamide adenine dinucleotide phosphate (NADPH; 400 μM) and HMG-CoA (400 μM) were present in the reaction mixture, which was mixed with 200 μl of potassium phosphate buffer...
品牌:Sigma-Aldrich 是否进口:是 [Sigma-Aldrich]抗CTCF抗体|11 zinc finger transcriptional repressor价格:￥1008.6/瓶产品详情:CTCF是一种普遍使用的11锌指(ZF)蛋白,具有高度通用的功能。转录阻遏物,CTCF,可与脊椎动物c-myc基因的启动子结合。它还与PLK和... ...
OA (Sigma-Aldrich) and PA (Sigma-Aldrich) were dissolved in dimethyl sulfoxide (Sigma-Aldrich). A cell stimulation cocktail (Invitrogen, Carlsbad, CA, USA) was added for 18 h to stimulate the lymphocytes. The cells were then treated with 10 ng/mL recombinant CXCL11 (rCXCL11) (R&D ...
Anhydrous and general grade solvents were purchased from the Sigma-Aldrich Chemical Co. and used without further purification. Water for aqueous solutions was obtained from a Nanopure Diamond system (18.2 MΩ.cm−1). Human recombinant AMACR 1A was expressed and purified and Funding sources This ...
NADPH-dependent acetoacetyl-CoA reductase activity was assayed in the mixture composed of 200 μM NADPH, 1 to 20 μM acetoacetyl-CoA (Sigma-Aldrich, St. Louis, MO, USA), and enzyme solution with appropriate dilution in 200 μL of 100 mM Tris–HCl buffer (pH 8.0). The consumption of ...
The cells grown on coverslips were fixed with 4 % paraformaldehyde (PFA; Sigma-Aldrich) for 10 min. After washing three times in ice-cold 1 × PBS, the cells were blocked with 3 % normal goat serum (NGS; Vector Laboratories, Newark, CA, USA) and incubated with anti-myosin skelet...
Myristoyl-CoA lithium salt (Sigma-Aldrich, M4414) MM = 977.9 g/mol; 5 mg diluted in 25.565 mL. Stock solutions (0.2 mM) are prepared in 10 mM sodium acetate, pH 5.6, and 1% Triton X-100, except for MALDI analysis, where cholate is used instead of Triton to reduce background (see...
Differentiation Markers Mouse Anti-AFPRabbit Anti- αSMAMouse Anti- TUBB3 1:1001:1001:100 Sigma-Aldrich Cat# A8452, RRID:AB_258392Abcam Cat# ab124964, RRID:AB_11129103BioLegend Cat# 801202, RRID:AB_10063408 Secondary antibodies Alexa flour 594 donkey anti rabbitAlexa flour 488 donkey anti rab...
Cells expressing the plasmid yielding the loading domain were resupended in Buffer C, processed as described above and purified over a pre-equilibrated 1 mL StrepTrap column (GE Healthcare), using 2 mM d-Desthiobiotin (Sigma-Aldrich) for elution. Fractions were tested on an SDS-PAGE gel, ...