Colocalization experiments between caveolin-1-EGFP and TRPV1-RFP inside cells.Barbara StortiCarmine Di RienzoFrancesco CardarelliRanieri BizzarriFabio Beltram
In addition, co-localization experiments indicated that glutamate like immunoreactivity was present in only two-thirds of AATase-like immunoreactive neuronal profiles. These findings suggest that glutaminase may be a more reliable marker of glutamatergic function than AATase.doi:10.1002/cne.902470407...
The Co-IP and co-localization experiments suggest that PRPF31 interacted with PRPH2. Introduction Retinitis pigmentosa (RP) is a group of inherited disorders characterized by photoreceptor and retinal pigment epithelium dystrophy [1]. The patients with RP usually complain of trouble seeing at night...
In the co-localization experiments, when PRL staining using AEC as chromogen was followed by GH staining using Vector SG as chromogen, a significant number of double-labeled acidophils were seen. If either antibody detection order or chromogen order was reversed, identification of double-labeled ...
Recently, a large number of fluorescent dyes with distinct fluorescence excitation and emission spectra have been engineered to be used in multilabeling and co-localization experiments. Some of the most common fluorescent dyes are fluorescein isothiocyanate (FITC), Cy2, Cy3, Cy5, TRITC, and ...
Quantitative colocalization studies suffer from the lack of unified approach to interpret obtained results. We developed a tool to characterize the results of colocalization experiments in a way so that they are understandable and comparable both qualita
experiments,thevolumeofinterestmaybethesamecell–arethetwoproteinsexpressedinthesamecell? Ifthisisthecasethenthereisnobetterreplacementformanualcountingof“double-positivecells”.For tissues,stereologicalapproachestodeterminenumberswouldbeadvised. Inothercaseswemaywanttoknowifthetwoproteinsoccurinthesameprotein-comple...
The subtype selectivity of the compound used in these experiments, DC- 32– 87, is somewhat controversial, and other groups have reported that this compound has high affinity for sst2, -3, and -5, which might explain the differences in our results...
RNA interference experiments reveal that endogenous mDia1 knockdown in HeLa cells results in the loss of PKD2 from mitotic spindles and alters intracellular Ca(2+) release. Our results suggest that mDia1 facilitates the movement of PKD2 to a centralized position during cell division and has a ...
This localization and the results of immunoprecipitation experiments suggest that the CD59 gene could encode the MIC11 antigen; alternatively this region of 11p13 may contain a cluster of genes encoding cell surface molecules. 展开 关键词: basic biological sciences human chromosomes genetic mapping ...