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The concentration of nitric oxide (NO) in the culture medium was measured using the Griess reagent method (lot no. S0021S; Beyotime, China), according to the manufacturer's protocol. Absorbance at 540 nm was measured using a microplate reader (Sigma Aldrich, St. Louis, MO, USA). The ...
It helps to bend the membrane to create a spherical structure encircling the ribonucleoprotein and serves as a scaffold for viral assembly [15]. Notably, the generation of infectious spherical particles is promoted by co-expression of the E and M proteins [11]. The N proteins predominantly...
go-sigma/cosignPublic forked fromsigstore/cosign NotificationsYou must be signed in to change notification settings Fork0 Star0 Files dev .github cmd doc hack images internal pkg release scripts specs test .gitattributes .gitignore .golangci.yml ...
As the world continues to experience the COVID-19 pandemic, seasonal influenza remain a cause of severe morbidity and mortality globally. Worse yet, coinfection with SARS-CoV-2 and influenza A virus (IAV) leads to more severe clinical outcomes. The devel
·mL−1, Sigma) was slowly added to the lipid solutions with vortexing until the solution was free of turbidity. The apoE solution was then added and the mixture vortexed three times for 10 min, at 5 min intervals and left to stand for 1 h. Then the entire solution was ...
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USA) according to the manufacturer’s manual. After the electroporation procedure, the cells were incubated for 6 hours at 37°C, 5% CO2 for attachment, and then the medium was replaced with OptiPro SFM with 1x GlutaMax (Gibco), 1x antibiotic-antimycotic and 2.5 μg/mL trypsin (Sigma, ...