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Suspend cell pellets in 1 mL of 1x wash buffer +spermidine+ protease inhibitor cocktail (PIC) by gentle pipetting. Cell pellets were washed three times with the same buffer. About 10 μL of activated Concanavalin A magnetic beads were incubated with 100 μL of prepared cells at room...
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Currently, coronavirus disease 2019 (COVID-19) is still spreading in a global scale, exerting a massive health and socioeconomic crisis. Deep insights into the molecular functions of the viral proteins and the pathogenesis of this infectious disease are
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barrier function by promoting epigenetic and transcriptional expression of protein channels at the alveolar capillary membrane that regulate alveolar fluid clearance, which include aquaporin-5, cystic fibrosis transmembrane conductance regulator, epithelial sodium channel, and the Na+/K+-ATPase...
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The distinction between the direct, immediate virus-induced (cardiotropic) cytotoxicity and the indirect, delayed virus-associated, immune-mediated (vasculopropic and lymphotropic) inflammatory cardiomyopathy has been discussed [201]. The numerous viruses include adenoviruses, enteroviruses (e.g., ...
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