EUKARYOTIC EXPRESSION The CMV promoter is considered to be a strong promoter and to function in many different cell lines.18 However, expression in eukaryotic cells is sensitive to many factors. If little or no expression is observed in eukaryotic cells, several factors can be considered. ♦ ...
Human cytomegalovirus (HCMV) latency and reactivation is regulated by the chromatin structure at the major immediate early promoter (MIEP) within myeloid cells. Both cellular and viral factors are known to control this promoter during latency, here we will review the known mechanisms for MIEP ...
2) promoter/non-promoter 启动子/非启动子3) promoter [英][prə'məʊtə(r)] [美][prə'motɚ] 启动子 1. A DNA Autonomous Replication Origin with Promoter Function in Yeast; 一个具有启动子功能的酵母DNA自主复制起始区 2. Construction of recombinant of luciferase promoter ...
12. An expression vector comprising the isolated nucleic acid of claim 8 operably linked to a promoter. 13. An isolated host cell comprising the expression vector of claim 12. 14. A pharmaceutical composition comprising the antibody or antigen binding fragment of claim 1 and a pharmaceutically ac...
Promoter sequence analysis showed that CmVDAC promoter sequence contains cis-acting elements that regulate plant growth and development and stress response element, suggesting that CmVDAC may be invloved in palnt growth and development and stress response. Tissue-specific expression showed that CmVDAC ...
www.nature.com/scientificreports OPEN received: 30 July 2015 accepted: 22 October 2015 Published: 19 November 2015 CMV promoter mutants with a reduced propensity to productivity loss in CHO cells Benjamin Moritz1, Peter B. Becker2 & Ulrich Göpfert1 The major immediate-early promoter ...
AbstractAlthough the functions of programmed death-1 (PD-1) on αβ T cells have been extensively reported, a role for PD-1 in regulating γδT cell function is only beginning to emerge. Here, we investigated the phenotypic and functional characteristics of PD-1-expressing γδT cells, and...
Promoter: As used herein, the term “promoter” may refer to any of a number of nucleic acid control sequences that directs transcription of a nucleic acid. Typically, a eukaryotic promoter includes necessary nucleic acid sequences near the start site of transcription, such as, in the case ...
The major immediate-early promoter and enhancer of the human cytomegalovirus (hCMV-MIE) is one of the most potent DNA elements driving recombinant gene expression in mammalian cells. Therefore, it is widely employed not only in research but also in large
Any suitable vector may be used in DNA vaccine compositions according to the invention. For example, pMG vector (Invitrogen) has been developed with two different promoters, one of which is the elongation factor 1α/HTLV hybrid. This is a strong promoter suitable for use with the DNA vaccines...