1.10 实时荧光定量PCR 提取叶片RNA并进行纯度和浓度检测, RNA 反转录采用PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (RR047A)试剂盒(TaKa- Ra宝生物工程有限公司, 中国大连).使用Primer- Quest Tool设计引物, 以ACTIN2基因为内参, 引物 任凯丽等: 甜瓜CmVDAC基因家族的鉴定及转录分析...
Purified PCR product was then submitted for Sanger sequencing in both directions using same primers as PCR (GeneWiz, South Plainfield, NJ). Sequence results were blasted against the IMGT mouse databank of germ-line genes using V-Quest (http://imgt.org) and specific 50 and 30 primers were ...
The RT-PCR primers were degenerate for the amplification of a 322–328 bp region in ULBP1, ULBP2, and ULBP3 (corresponding to the major hybridizing band in each lane). The integrity of the cDNAs was evaluated by PCR by using HPRT, G3PDH, or S9 ribosomal protein primers as indicated ...
Uninfected HMECs and HCMV-DB-infected HMECs at low and high MOIs (1 or 10) were used one day after infection for the screening with oncogenes/tumor suppressor genes and human breast cancer genes RT2 profiler PCR assays (PAHS-502Z and PAHS-131Z, respectively). Positive controls included ...
From 5 million B cells surveyed, drawn from 48 donors, with an emphasis on the 4 donors who had the highest titer to gB (AD-2), 30 mAbs were cloned by single-cell cDNA PCR and expressed in HEK293 cells. Based on superior potency for neutralization of HCMV in vitro [83], TRL345 ...