CMV promoterCytomegalovirus (CMV) immediate early promoter is a powerful promoter frequently used for driving the expression of transgenes in mammalian cells. However, this promoter gradually becomes silenced in stably transfected cells. We employed Chinese Hamster Ovary (CHO) and human pancreatic cancer...
2.SOX2通过抑制PML-NBs的形成来调节HCMV感染 由于有报道SOX2直接结合人乳头瘤病毒E6上游调控区下调癌基因E6和E7的表达,我们首先推测SOX2可能通过与HCMV主要直接早期启动子(major immediate early promoter, MIEP)结合的类似机制来增加HCMV基因...
1) minimal cytomegalovirus immediate-early promoter 最小CMV启动子 2) CMV promoter CMV启动子 1. Objective To study the effect of ionizing radiation on the expression of liposome mediated GFP reporter gene drived by EGR - 1 gene promoter andCMV promoter, respectively in hepatoma 7402 cells. ...
www.nature.com/scientificreports OPEN received: 30 July 2015 accepted: 22 October 2015 Published: 19 November 2015 CMV promoter mutants with a reduced propensity to productivity loss in CHO cells Benjamin Moritz1, Peter B. Becker2 & Ulrich Göpfert1 The major immediate-early promoter ...
Eukaryotic expression is driven by the cytomegalovirus (CMV) immediate early promoter in the pBK-CMV phagemid vector. Stable clone selection in eukaryotic cells is made possible with G418 by the presence of the neomycin- and kanamycin-resistance gene, which is driven by the SV40 early promoter ...
3)minimal cytomegalovirus immediate-early promoter最小CMV启动子 4)CMV enhancerCMV增强子 1.Methods CMV enhancer,CEA promotor and cd tk fusion gene were obtained by PCR.方法 PCR法分别扩增出CMV增强子 ,CEA启动子 ,cd tk ,构建真核表达载体 pE CEA cd tk ;MTT法检测pE CEA cd tk/5 FC +GCV体系的...
CMV promoter Human cytomegalovirus (CMV) immediate early enhancer and promoter. Open in SnapGene Try SnapGene for Free Download Plasmid|Download SnapGene Viewer Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets HomePlasmidsBasic...
Eukaryotic expression is driven by the cytomegalovirus (CMV) immediate early promoter in the pBK-CMV phagemid vector. Stable clone selection in eukaryotic cells is made possible with G418 by the presence of the neomycin- and kanamycin-resistance gene, which is driven by the SV40 early promoter ...
The pBK-CMV phagemid vector1 is a cloning vector derived from a high-copy-number pUC-based plasmid. This vector allows expression in both eukaryotic and prokaryotic systems. Eukaryotic expression is driven by the cytomegalovirus (CMV) immediate early promoter in the pBK-CMV phagemid vector. Stable...
Ward CM, Stern PL: The human cytomegalovirus immediate-early promoter is transcriptionally active in undifferentiated mouse embryonic stem cells. Stem cells. 2002, 20: 472-475. 10.1634/stemcells.20-5-472. Article CAS PubMed Google Scholar Caron L, Bost M, Prot M, Hofman P, Binétruy B: ...