EDIP97 and CML2001 are both midpoint approaches and hence quite similar in their scope and structure, and this allows a comparison during both characterisation and normalisation. The third impact assessment method Eco-indicator 99 is an endpoint method and different in scope and structure from the...
The limitations of that method are a lack of universal availability of the test or the achievement of a remission that is already too deep to detect the BCR::ABL1 fusion by FISH. With respect to test availability, dried, unstained bone marrow smears stored frozen (−18 °C) can be ...
Quantitative nested PCR allowed the detection of residual BCR-ABL transcripts in all complete cytogenetic responders on IgN-α. We conclude that competitive PCR with internal controls is a reliable method for monitoring patients on IFN-α and reduces the need for repeated marrow investigations. 展开 ...
Total viable cells were assessed on a Neubauer hemacytometer (Hausser Scientific, Horsham, PA, USA) using the trypan blue (Life Technologies) exclusion method. Apoptosis analysis was performed using an Apoptosis Detection kit (Thermo Fisher Scientific). After being treated with inhibitors for 24 or ...
The ProgramCallDocument class contains the transformPCMLToXPCML method that enables you to transform existing PCML documents to equivalent XPCML documents.
The function is described in words (in this case the method name is sufficient) and we implement a test which runs the code against an expected valid output: @Test public voidtestGetAverageBondLength() { molTool5.calculateBondedAtoms(); ...
To assess the sensitivity of the method to detect a clonal population in a background of polyclonal cells, (polyclonal) CD3+cells and (monoclonal) CD33+ cells from a CML patient with 100% Ph+ metaphases were mixed at graded concentrations, and the cell mix subjected to HUMARA-PCR (Figure1...
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods., 25 (2001), pp. 402-408 View PDFView articleGoogle Scholar 9 NC Cross, L Feng, A Chase, J Bungey, TP Hughes, JM Goldman Competitive polymerase chain reaction to ...
Relative gene expression was calculated using the 2-ΔΔCT method. Mixed lymphocyte reactions and CD107a expression assay Peripheral blood mononuclear cells (PBMCs; responder cells) were isolated from healthy donors by density centrifugation, using Histopaque (Sigma-Aldrich, Poole, UK). The PBMCs ...
are produced. The former method not only has technical difficulties related to in preparation but also is less effective than the latter in vivo [59]. Thus, for the preparation of indirectly antigen-loaded Dex, we first loaded DCs with CML tumor cell lysates to generate CML-specific DCs. Thi...