clusterCells(cds=, num_clusters=, method=) num_clusters=: 结果中的聚类数目;结果中的数目为此数字减一 method=:"densityPeak"/"louvain"/"DDRTree"; 默认是"densityPeak" Clustering 结果可以通过pData(Monocle2_object)方式查看 展示降维和聚类结果:plot_cell_clusters(cds, color_by=)...
第四步 使用训练器进行注释 #读取marker filehsPBMC<-readRDS("hsPBMC.rds")pData(cds)$garnett_cluster<-pData(cds)$seurat_clusterscds<-classify_cells(cds,hsPBMC,db=org.Hs.eg.db,cluster_extend=TRUE,cds_gene_id_type="SYMBOL")#提取分类结果cds.meta<-subset(pData(cds),select=c('cell_type',...
Antigen distribution on hematopoietic stem cells, erythrocytes, epithelial cells, endothelial cells, natural killer (NK) cells, monocytes/macrophages, T cells, B cells, granulocytes, megakaryocytes/platelets, and dendritic cells are illustrated graphically. The chapter also provides information regarding ...
cells (HSC) derived from fetal liver of Em-myc transgenic mice expressing miR-17/19 under the control of murine stem cell virus (MSCV) show a massive enlargement of lymph nodes, splenic hyperplasia, infiltration of the thymus by lymphoma cells and leukemias.13 Moreover, almost half of the ...
At room temperature, single CdSe/CdS core/shell gNCCs with a shell thickness of 20 MLs were excited by a 405 nm pulsed laser (see Methods), whose fluorescence spots can be clearly seen from the confocal scanning PL image shown in Fig.2a. The PL spectrum of a single gNCC is shown in...
samp_cds <- cluster_cells(samp_cds, k=ceiling(sqrt(dim(samp_cds)[2])*0.75)) } # Increase number of clusters if clusters are < 5 if (dim(table(clusters(samp_cds))) < 5 ) { samp_cds <- cluster_cells(samp_cds, k=ceiling(sqrt(dim(samp_cds)[2])*0.1)) ...
4G and Supplementary Movie 7, MC-CF@PEM-RGD floated along the direction of blood flow before a magnetic field was applied; however, with the application of a magnetic field, MC-CF@PEM-RGD was able to swim through flowing red blood cells, which supported the above-mentioned results. These ...
Efficient Sensitization of Dye-Sensitized Solar Cells by Novel Triazine-Bridged Porphyrin–Porphyrin Dyads Two novel porphyrin-porphyrin: dyads, : the symmetrical Zn[Porph] Zn[PorPh] (2) and unsymmetrical Zn [Porph]-H-2[PorPh] (4), where Zn[Porph] and H-2[Porph]... GE Zervaki,MS...
TNB I26 ectB gene for diaminobutyric acid aminotransferase, partial cds To extract carotenoids from Thraustochytrids Characterize them and examine their effects on human cells. T Okamoto,T Naganuma 被引量: 0发表: 2003年 Cloning of the ectoine biosynthesis gene ectABC from Halomonas sp.BYS-1 ...
这里是把seurat对象转换为了cds对象; erythroid.cds<-as.cell_data_set(erythroid)erythroid.cds<-cluster_cells(cds=erythroid.cds,reduction_method="UMAP")erythroid.cds<-learn_graph(erythroid.cds,use_partition=TRUE)#这个教程是已经手动提供了root的细胞,如果没有这个文件,order_cells()会开一个窗口,然后让人...