3.3 Safety Data Sheet (SDS) In addition to the label, another key tool for hazard communication in the supply chain, intended for professional/industrial users only, is the SDS. The SDS format and content as an integral part of the EU REACH is fully in line with CLP, both maintaining 16...
Currently, the classification rules, mandatory classification list as well as the format of SDS under GB CLP and EU CLP are highly consistent. However, as time goes on, there may be some amendments to these regulations and the classifications of the same substances/mixtures may be different. It...
Then, obtained protein lysates were degenerated at 100 °C for 5 min, and separated by 10% SDS–PAGE and electrophoretically transferred onto polyvinylidene fluoride membranes (Millipore Corp, Bedford, MA). The membranes were blocked with 3% BSA for 1 h at room temperature, then ...
called“ElectrostaticallySensitive(ES)Devices”orESDs.ExamplesoftypicalESDsare:integratedcircuits, somefieldeffecttransistors,andsemiconductor“chip”components. Thetechniquesoutlinedbelowshouldbefollowedtohelpreducetheincidenceofcomponentdamage causedbystaticelectricity. CautionBesurenopowerisappliedtothechassisorcircuit...
Affinity eluates of the transgenic lines expressing CLPC1-WT-STREP and CLPC1-TRAP-STREP were separated by SDS-PAGE on Biorad Criterion Tris-HCl precast gels (10.5%–14% acrylamide gradient) with three biological replicates. We refer to these eluates further as CLPC1-WT and CLPC1-TRAP. Each...
At each of the time, the reaction products were resolved on 12% SDS-PAGE and visualized by Coomassie staining. A similar procedure was followed for the β-casein proteolysis assays wherein mutant isoforms of LepClpP (LepClpP1S98A and LepClpP2S97A) were used. In an alternative assay format...
CLP classification and labelling info must be included in Safety Data Sheets. However, SDSs are governed by theREACH regulation. The Annex II of REACH has set out detailed requirements on the format and content of SDSs. Any new SDSs compiled or existing SDS updated after 1 June 2015 must ...
For immunoblotting, native PAGE gels were incubated for 1 h in transfer buffer (25 mM Tris, 192 mM Glycine, 15% v/v methanol, 0.5% w/v SDS) and subsequently transferred to a PVDF membrane (Merck) via semi-dry protein blotting at 0.8 mA/cm2starting voltage for approx. 80...
Proteins were separated via SDS-PAGE58, transferred to a PVDF membrane (Immun-Blot, Bio-Rad, Munich, Germany) and incubated with one of the following primary antibody diluted in 5% BSA in PBS overnight at 4 °C: β-actin (mouse, 1:1000/Cat. #MAB1501, Merck, Darmstadt, Germany), Casp...
Mass spectrometry and nano-LC settings for the spectral counting experiment were the same as described in detail above (see “Label-free Shotgun Analysis by 1-D SDS-PAGE and Nano-LC-LTQ-Orbitrap Mass Spectrometry of Seedlings”). Quantification of clpr2-1/wt ratios was similar to the ...