scindens ATCC 35704 in addition to a partially purified steroid-17,20-desmolase (SDase); however, the genes encoding these enzymes have yet to be identified (7, 8). In mammalian cells, side-chain cleavage of glucocorticoids proceeds by an oxygen-dependent P450 monooxygenase reaction (9). ...
ATCC35704价格:3200元 代购价格:询价 联系方式:I47-825O-882O 相关技术服务:微生物基因敲除和回补立刻购买 买家导航 ATCC35704标准菌株基本信息 出品公司: ATCC 菌种名称: ATCC35704, ATCC 35704 菌种又名: VPI 13733 菌株类型: 闪烁梭菌,Clostridium scindens 存储人: LV Holdeman 分离来源: 人类粪便,纽约 产品...
C. scindens (ATCC35704) obtaining from Bioresource Collection and Research Center, Hsinchu, Taiwan, was cultured on Chocolate Agar II plate (BD Biosciences, USA) at 37 °C under anaerobic condition. After 48 h of incubation, the bacteria were expanded in 10 ml anaerobic Brain Heart Infusion ...
The desA and desB genes are annotated as N-terminal and C-terminal transketolases, respectively. We hypothesized that the DesAB forms a complex and has steroid-17,20-desmolase activity. We cloned the desA and desB genes from C. scindens ATCC 35704 in pETDuet for overexpression in ...
Clostridium scindens is a keystone bacterial species in the mammalian gut that, while low in abundance, has a significant impact on bile acid and steroid metabolism. Numerous studies indicate that the two most studied strains of C. scindens (i.e. ATCC 35704 and VPI 12708) are important for ...
BaiJ and BaiB are key enzymes in the chenodeoxycholic acid 7α-dehydroxylation pathway in the gut microbe Clostridium scindens ATCC 35704doi:10.1080/19490976.2024.2323233Clostridium scindensbile acids7α-dehydroxylationgut microbesbai geneschenodeoxycholic acid...
Identification of a gene encod- ing a flavoprotein involved in bile acid metabolism by the human gut bacterium Clostridium scindens ATCC 35704. Biochim Biophys Acta - Mol Cell Biol Lipids. 2018;1863:276-283. doi:10.1016/j.bbalip.2017.12.001....
Clostridium scindensA genetic Fluorescence Resonance Energy Transfer (FRET) detector undergoes a post-translational modification (PTM)-induced conformational change that results in increased FRET. To test if the PTM-dependent FRET change can be quantified by flow cytometry, we purified and immobilized a...
scindens ATCC 35704 SteroidTrivial NameRelative Activity % 4-PREGNEN-11β, 17, 21-TRIOL-3, 20-DIONE CORTISOL 100.00 ± 2.78a 4-PREGNEN-17, 21-DIOL-3, 20-DIONE 11-DEOXYCORTISOL 186.73 ± 3.60 4-PREGNEN-11α, 21-DIOL-3, 20-DIONE 11-EPICORTICOSTERONE NAb,c 4-PREGNEN-11β, 21-DIOL-3...
scindens growth (Figures 1A and 1B). Next, we decided to grow C. difficile ATCC 9689 (type strain) and C. scindens ATCC 35704 in PYF medium in co-culture with and without added CA (50 μM). To measure the growth of each bacterium, we developed specific PCR assays to quantify a ...