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Isolation and characterization of the organ-specific and light-inducible promoter of the gene encoding rubisco activase in potato (Solanum tuberosum) Constitutive promoters have been widely used in crop biotechnology applications. Tissue-specific or inducible promoters, however, have advantages in some c....
- 《Bioscience Biotechnology & Biochemistry》 被引量: 39发表: 2006年 Study of Putative Glycosylation Sites in Bovine β-Casein Introduced by PCR-Based Site-Directed Mutagenesis The bovine β-casein gene, A2 genetic variant, has been mutated at positions 70 and 71 for the introduction of a ...
Engineering Perspectives in Biotechnology M.Pyne, ...C.P.Chou, inComprehensive Biotechnology (Third Edition), 2014 2.03.2Molecular Cloning and Recombinant DNA Technology Molecular cloningrefers to a variety of techniques inmolecular biology, collectively known asrecombinant DNAtechnology, utilized to target...
The lux genes of Ph. luminescence are suggested for use in gene engineering and biotechnology. 展开 关键词: genes bioluminescence biotechnology chromosomes DNA nucleotide sequences recombination vectors symbionts DOI: 10.1097/00041444-200010010-00010 ...
in Sepiella maindroni has ten specific amino acid residues:Ile12,Ser172,Ser174,Gln223,His227,Ile231,Gly232,Ser320,Glu328 and Thr360,respectively.In addition,two other particular base sites of amino acid residues were found in these sequences.Similarly,two characteristic amino acid residues of ...
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In a wild type Ti plasmid, most of the T-DNA, which is transferred to the plant cells, is fairly large (~25 kb), does not contain useful restriction sites for cloning, and has been found to be nonessential for g...
- 《World Journal of Microbiology & Biotechnology》 被引量: 34发表: 2015年 Purification and biochemical characterization of manganesecontaining superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3 Cloning and characterization of a new manganese superoxide dismutase from deep-sea thermophile ...
Trends in biotechnology 23, 199–207, doi: 10.1016/j.tibtech.2005.02.008 (2005). Article CAS PubMed Google Scholar Tillett, D. & Neilan, B. A. Enzyme-free cloning: A rapid method to clone PCR products independent of vector restriction enzyme sites. Nucleic Acids Research 27, e26–e28...