We chose a beautiful curio cabinet. Our rather remote location precluded delivery. However, The Clock Depot was Most helpful in locating an appropriate dealer near us. We are able to have this exquisite piece of furniture after all! Furthermore, he made special effort to locate clock repairmen...
For the Dh44-R1 mutation, a homology directed repair template (HDR) was also used. 5′ and 3′ homology arms spanning 1 Kb upstream and downstream of the desired deletion were cloned into the pHD-DsRed-attP plasmid [66]. Guide RNAs and HDR template were injected into vasa-Cas9 flies ...
Fig. 4 The classic cellular information flow from DNA to RNA and to protein is subject to circadian control at all levels. This rhythmic biological information flow contributes to the circadian control of cellular physiology and downstream biological processes. Among these, we highlight DNA repair, ...