TMR red (Merck, cat no. 12156792910) following the manufacturer’s instructions, adapted to screen organoid sections. Briefly, organoid sections were permeabilized with 0.1% Triton-X in 0.1% sodium citrate for 8 min at 4 °C. After 3x wash with PBS, sections were incubated with...
(Sigma-Aldrich, cat no. AMPD1-1KT, 1unit/uL) for 10 min at RT to induce DNA strand breaks. Negative controls were incubated with label solution only. Following 3x wash with PBS, sections were mounted with Fluoromount-G mounting medium (Southern Biotech, cat no. 0100–01) on a glass...