用于流式细胞分析的 Click-iT EdU 细胞计数、细胞增殖和细胞周期试验 —第 15.4 节 用于流式细胞分析的细胞增殖试验 介绍 检测细胞增殖能力是评估细胞健康状况和基因毒性以及评价抗癌药物的基本方法。最准确的方法是直接检测 DNA 的合成。最初,...
通过补料或注射方法给予 EdU 后,Click-iT™ 测定试剂盒可用于培养的细胞或体内的细胞。 通过使用不与 EdU 发生交叉反应的抗 BrdU(克隆 MoBu-1)抗体,可将 Click-iT™ 测定试剂盒与 BrdU 一起用于双脉冲实验。 Click-iT™ 技术与具有固定耐受性或设计用于固定细胞标记的免疫组织化学、免疫细胞化学以及荧光染...
通过补料或注射方法给予 EdU 后,Click-iT™ 测定试剂盒可用于培养的细胞或体内的细胞。 通过使用不与 EdU 发生交叉反应的抗 BrdU(克隆 MoBu-1)抗体,可将 Click-iT™ 测定试剂盒与 BrdU 一起用于双脉冲实验。 Click-iT™ 技术与具有固定耐受性或设计用于固定细胞标记的免疫组织化学、免疫细胞化学以及荧光染...
This EdU assay kit can also be multiplexed with antibodies against surface and intracellular markers.Quick and Simple Protocol The Click-iT® EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling, but EdU is compatible with other...
The Click-iT® EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling, but EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proli...
Detection of the proliferating cells withinthe tissue section requires DNA denaturation (typically using HCl, heat, or digestionwith DNase) to expose the BrdU so that it may be detected with an anti-BrdU antibody (Figure 1, left panel). However, the denaturation step for the BrdU protocol can...
含有EdU(5-乙炔基-2'-脱氧尿苷)、Alexa Fluor™ 染料-吡啶甲基叠氮化物、无水二甲基亚砜 (DMSO)、Click-iT™ EdU 反应缓冲液、铜保护剂、Click-iT™ EdU 缓冲液添加剂和 Hoechst 33342。 储存于 2°C 至 8°C 干燥避光储存。 图表 Comparison of Click-iT EdU and Click-iT Plus EdU ...
The Click-iT® EdU Alexa Fluor® 488 HCS Assay provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT® EdU, mild fixation...
图2.Alexa Fluor® 488 Click-iT® Plus EdU Flow Cytometry Assay Kits, mCherry, and FxCycle™ FarRed A549 cells were transduced with adenovirus for 48 hours, treated with 10 µM EdU for 2 hours and detected according to the recommended staining protocol. The figures show a clear separ...
图2.Alexa Fluor® 488 Click-iT® Plus EdU Flow Cytometry Assay Kits, mCherry, and FxCycle™ FarRed A549 cells were transduced with adenovirus for 48 hours, treated with 10 µM EdU for 2 hours and detected according to the recommended staining protocol. The figures show a clear separ...