Click-iT EdU 细胞增殖成像试剂盒是一种优于传统细胞增殖检测的替代方法,其针对荧光显微镜应用进行了优化。在该检测中,将经修饰的胸苷类似物 EdU 有效地掺入新合成的 DNA 中,并在快速、高度特异性的click了解更多信息 Have Questions? 货号C10339 价格(CNY)/ Each ...
不同于使用 BrdU 染色的测定法,Click-iT EdU 测定法不基于抗体,因此并不需要 DNA 变性以检测掺入的核苷。此外,为了提高实用性,Click-iT Plus EdU 测定法可以配合 R-藻红蛋白 (R-PE) 和 R-PE 串联体、荧光蛋白 (GFP 和 mCherry)以及 还在 BrdU和 EdU 双重染色实验中配...
The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions. Standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click‑iT® detection reagent to gain access to the DNA. This is in contrast to BrdU assays that ...
3.2. In Vitro Antiproliferative Assays and Cellular Uptake The human HCT116 colorectal carcinoma, MCF7 breast adenocarcinoma, A549 non-small cell lung carcinoma, and WI38 nonmalignant lung fibroblast cell lines were obtained from the European collection of authenticated cell cultures (ECACC; Salisbury,...
Detection Principle Cell proliferation assays are widely used in the evaluation of cell viability, genotoxicity, and the effect of antitumor drugs. Direct detection of DNA synthesis in cells is considered to be the most accurate method for detecting cell proliferation. The initial widely used...
规格:50 assays 价格:8119 物种:其它 宿主:其它 抗体亚型:其它 荧光染料:其它 产品介绍 技术参数 相关产品 产品介绍 描述 The Click-iT® Plus EdU Pacific Blue™ Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells compared to traditiona...
Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution as well as the destruction of antigen recognition sites when using the acid denaturation method. In contrast, the Click-iT™ Plus EdU cell proliferation kit is compatible with cell cycle dyes, R...
53 These multipotent SCs differentiate into cell types from the germ layer of their origin. However, it has been found that they are generally less effective than ESCs in generating other lineages (Figure 1A). Today, it is widely agreed that several subsets of SCs can be found in the BM:...
The Invitrogen Click-iT EdU cell proliferation assays combine EdU (5-ethynyl 2´-deoxyuridine) labeling with click chemistry to provide an alternative to traditional BrdU staining methods for detecting and quantitating newly syn...
DNA synthesis–based cell proliferation assay In this assay, the modified thymidine analog EdU (5-ethynyl-2′-deoxyuridine, a nucleoside analog of thymidine) is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor® d...