Caspase-3是一种半胱氨酸-天冬氨酸蛋白酶,在细胞凋亡过程中起到关键作用,被广泛用作凋亡的生物标志物。Caspase-3在凋亡过程中,被剪切成分子量分别为17kDa(p17)和12kDa(p12)的两个片段,并易位至细胞核。Caspase-3经过剪切激活后,可以激活 caspase-6、-7 和 -9,形成
120min、150min都能转出来,关键是即使诱导凋亡,细胞内剪切的caspase3含量依旧比较低,所以一个是提蛋白...
Caspase-3正常以酶原(32KD)的形式存在于胞浆中,在凋亡的早期阶段,它被激活,活化的Caspase-3由两个大亚基(17KD)和两个小亚基(12KD)组成,裂解相应的胞浆胞核底物,最终导致细胞凋亡。但在细胞凋亡的晚期和死亡细胞,caspase-3的活性明显下降。 应用类型:WB,IHC/ICC,IP. 免疫原:抗体类别为IgG,包含磷酸盐缓冲液...
WB,IHC/ICC,IP 免疫原 抗体类别为IgG,包含磷酸盐缓冲液和0.01%的叠氮钠。 来源宿主 小鼠或人 Caspase 3 p18亚基羧基端的13个氨基酸为抗原。 反应性 能特异切割人和小鼠的 caspase 3 保存建议 在4度下保存一个月,分装保存-20度可保存更长时间,避免反复冻融。
cleaved-Caspase 3 Print datasheet MSDS Validation Data Gallery Filter: AllWBIPIHCIF WB analysis of HepG2 using 25546-1-AP Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperat...
Validated for WB, WB, IP, IHC, IF, F. Available in 3 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-3 (Asp175) Antibody (CST #9661) is ready to ship. 下单购买 下单前请务必联系店铺客服,提供全程优质服务。 查询产品...
Monoclonal Antibody for studying Casp3 (Asp175) cleaved. Validated for ELISA, F, IF, IHC, WB. Highly specific and rigorously validated in-house, Cleaved Caspase-3 (Asp175) (5A1E) Rabbit Monoclonal Antibody (BSA and ...
Caspase 3 (Cleaved Asp175) Antibody (PA5-114687) in WB Western blot analysis of Caspase 3 (Cleaved Asp175) in various samples (Lane 1: HeLa treated with blocking peptide Lane 2: HeLa (etoposide treated, 25uM 5h); Lane 3: MCF7 (etoposide treated, 25uM 5h); Lane 4: M...
货品编号: affinity.AF7022 品牌: affinity 品名: Cleaved-Caspase 3 (Asp175), p17 Antibody - AF7022 规格: 50ul 产品描述: Applications: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 Reactivity: Human, Mouse, Rat Predicted Reactivity: Pig(...
western时,测定cleaved caspase-3,total caspase-3和β-actin都要做。 请问您用的是哪家的caspase 3抗体呢 豆龟 楼主现在做的进展如何呢?我现在也是打算做斑马鱼蛋白的caspase 3的WB,之前用abcam的抗体做了,但没有出条带,请问你用的哪家的抗体? 神经再生 7楼: Originally posted by 豆龟 at 2012-11-28 12...