Caspase.3在正常包皮与尖锐湿疣组织中的表达 cleaved C嬲pase.3的阳性表达主要定位于胞浆与胞核中,以胞浆为主,着色 淡黄色至棕黄色不等,在表皮各层中均有表达,以棘层和颗粒层为主。cleaved Caspase.3在15例正常对照中无表达,阳性率为O%;34例CA中有13例表达阳 ...
Cleaved Caspase-3
In this regard, proteolytic cleavage of tau at the C-terminus could affect the physiological interaction of tau with microtubules. Proteolytic cleavage of tau’s carboxy-terminal tail has been observed at Asp421 generating a tau species lacking the last 20 amino acids [20]. Cleavage is performed...
T, Balmaceno-Criss M, Ciucci T, Xiao Q, Kelly MC, McGavern DB, Belkaid Y,et al.1:200),Bim(PE,cloneC34C5,12186,Cell Signaling,1:20), CleavedCaspase-3(Pacific Blue,cloneD3-E9,8788,Cell Signaling,1:10), Bcl2 (PE,clone3F11,563096,BD Bioscience, 5μl/test). ...
Applications Tested: This HLNC4 antibody has been pre-titrated and tested by flow cytometric analysis of staurosporine-stimulated Jurkat cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. (Refer to Protocol B: One step protocol for intracellul...
(Cleaved Asp175) in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114687) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with ...
Examination of early-phase TULV infection revealed that infected cells were refractory to apoptosis, as evidenced by the complete lack of cleaved caspase-3 (casp-3C) staining, whereas in non-infected bystander cells casp-3C was highly abundant. Interestingly, at later time points, casp-3C was...
Apoptosis was read out as nuclear fragmentation by PI staining of nuclei and fluorescence-activated cell sorter analysis. B, J16 cells, stably transduced with empty vector, c-FlipL, or dn FADD cDNA were treated with etoposide,γ-radiation, or anti-CD95 mAb, and apoptosis was read out as ...
Western blotting for procaspase-3 (Casp-3) shows the cleavage of caspase-3 induced by TNFa/CHX (10 ng/ml and 10 mg/ml). (b) BID KO MEFs expressing GFP, GFP þ 1-380, GFP þ BID, or GFP þ 1-380 þ BID were exposed to TNFa/CHX for 6 h. Apoptosis was measured as ...
Staining of the TauC3 antibody co-localized with MC-1, AT8, and PHF-1 within NFTs. Quantitative analysis indicated that roughly 90% of PHF-1-labeled NFTs contained caspase-cleaved tau. In addition, we documented the presence of active caspase-3 within plaques, blood vessels and pretangle ...