The GEO database (https://www.ncbi.nlm.nih.gov/geo/) was used to search and obtain the human ccRCC miRNA expression dataset GSE12105, GSE16441, and GSE37989, and mRNA expression dataset GSE53757. Differential expression of miRNA and mRNA between ccRCC samples and controls were determined us...
Background Clear cell renal cell carcinoma (ccRCC) is a highly invasive and metastatic subtype of kidney malignancy and is correlated with metabolic reprogramming for adaptation to the tumor microenvironment comprising infiltrated immune cells and immunomodulatory molecules. The role of immune cells in the...
Here, we perform snRNA-seq and snATAC-seq in 34 and 28 human clear cell renal cell carcinoma (ccRCC) specimens, respectively, with matched bulk proteogenomics data. By identifying 20 tumor-specific markers through a multi-omics tiered approach, we reveal an association between higher ...
Cell proliferation was assessed in vitro using the Cell Counting Kit-8 (CCK-8) and plate colony formation assays. Human ccRCC cell lines were harvested during their logarithmic growth phase and seeded into 96-well plates at a density of approximately 2,000 cells per well in 100 μl of cultu...
TotalSeq-A anti-human Hashtag 5 (AAGTATCGTTTCGCA) Biolegend RRID:AB_2750019; Cat# 394609 Biological samples Renal tumor and background kidney samples Oxford Radcliffe Biobank (ORB) Available on request Chemicals, peptides, and recombinant proteins DMEM/F12 Gibco Cat# 11320033 Advanced DMEM/F12 ...
ES-2 and TOV-21G cell lines of human ovarian clear cell carcinoma (OCCC) were acquired from the American Type Culture Collection (ATCC, USA). The cells were cultured with DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA), penicillin (100 U/mL), and streptomycin (...
Background Z-DNA-binding protein 1 (ZBP1) is a critical Z-DNA- and Z-RNA-binding protein. However, its diagnostic and prognostic significance, as well as its functions in clear cell renal cell carcinoma (ccRCC), are not well understood. Materials and methods Paired and unpaired differential...
Stanniocalcin-1: a novel molecular blood and bone marrow marker for human breast cancer. Clin Cancer Res. 2003;9:1427–35. CAS PubMed Google Scholar McCudden CR, Majewski A, Chakrabarti S, Wagner GF. Co-localization of stanniocalcin-1 ligand and receptor in human breast carcinomas. Mol ...
Background: Clear cell renal cell carcinoma (ccRCC) is the most common and metastatic type of renal cell carcinoma. Despite significant advancements, the current diagnostic biomarkers for ccRCC lack the desired specificity and sensitivity, necessitating the identification of novel biomarkers and elucidation...