Practice Problems for Molecular Biology, Session 1 DNA Structure, Classic Experimentssu:分子生物学实践中存在的问题,会议1 DNA结构,经典experimentssu帮助,DNA,DNA,for 文档格式: .pdf 文档大小: 161.11K 文档页数: 2页 顶/踩数: 0/0 收藏人数:
SolutionstoPracticeProblemsforMolecularBiology,Session1:DNAStructure,ClassicExperiments Question1 In1928,Griffithperformedaseriesofexperimentswhereheinfectedmicewithtwodifferentstrainsof pneumonia-causingbacteria(S.pneumonia):thesmooth/virulent,diseasecausingSstrainandtheharmless rough/non-virulentRstrain.Youhavethreeunk...
The results of the toe-printing assay confirmed that the presence of the MTI or MAI tripeptide sequences in the P site of the ribosome results in CHL-dependent stalling, whereas MFI tripeptide sequence is not conducive to ribosome stalling and can serve as a negative control. Experiments were ...
W.Z., and C.J. preformed the experiments including animal experiments and biochemical analysis. X.C., Yuan.L., Y.J., and Yang.L. contributed to the preparation of KXS extracts and chemical separation. X.S. and S.L. assisted in writing the introduction and discussion sections of the a...
Notably, N1 methylation shifted almost entirely to G37 in native-state SufB2, indicating that m1G37 is the dominant methylation product in cells. As a control, no inhibition of extension at G37 or G37a was observed for G37-state SufB2. Complementary kinetics experiments showed that the ...
All growth experiments were conducted at 30°C in YP media (1% Yeast extract and 2% Peptone) supplemented with various carbon sources as follows. Growth profiles in media containing different concentrations of glucose, galactose, glucose and galactose, or galactose supplemented with different concentrati...
Data are representative of n=2 individual experiments. Significance was determined using the two-tailed Student’s t-test, and data are expressed as mean±s.d. *P<0.05; **P<0.01; ***P<0.001. Full size image We further performed a detailed titration of IL-6 and hIL-6 in the scratch ...
The information can then be organized on a timeline, geographically (using GPS data), using contexts (automatically determined using time and location) such as "in a restaurant" or "at work", additional information such as keywords, folders and comments. ...
GEH designed and produced the mouse models, coordinated experiments, interpreted data, and helped draft the manuscript. KS conducted much of the characterization of the intermediate MSUD model. CF assisted with production of the mouse models, data collection, analysis, and interpretation. SW directed ...
Therefore, in initial experiments, a general protease substrate was used, to determine effect of SCCA1 and SCCA2 on the staphopain activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis The reaction of a serpin with a target serine protease results in formation of a covalent complex ...