Biologics: A Class of Medication Produced by Recombinant DNA TechnologyMuniba Safdar
At the time, mRNA was not pursued further as it is less stable than DNA, and the field focused on technologies based on pDNA and viral DNA. Nevertheless, since its discovery in 1961, mRNA has been the subject of consistent basic and applied research for various diseases (FIG. 1 (...
Extracellular domains of recombinant human and mouse αvβ1, αvβ3, αvβ5, αvβ6, αvβ8, and α5β1 integrin proteins (Supplemental Table S1) were puri- fied, biotinylated, and used as baits to screen for specific binders through several rounds of enrichment by mag- netic ...
42, No. 5 3059–3072 doi:10.1093/nar/gkt1323 c-Jun/c-Fos heterodimers regulate cellular genes via a newly identified class of methylated DNA sequence motifs Montse Gustems1,*, Anne Woellmer1, Ulrich Rothbauer2, Sebastian H. Eck3, Thomas Wieland3, Dominik Lutter4 and...
Transposons can also cause loss of function if they land in a promoter or coding region of a gene. Several families of transposases have been identified. They can be broadly divided into two groups: those that form a covalent intermediate with DNA, such as the serine, tyrosine, and rolling ...
Extracellular domains of recombinant human and mouse αvβ1, αvβ3, αvβ5, αvβ6, αvβ8, and α5β1 integrin proteins (Supplemental Table S1) were puri- fied, biotinylated, and used as baits to screen for specific binders through several rounds of enrichment by mag- netic ...
Cloning of constructs All yeast protein expression constructs were cloned from the S. cerevisiae Genomic DNA library (Novagen Cat. 69240-3) using the primers listed in Supplementary Data 2. The construct expressing the Msn5 protein was cloned into a pQE60 vector (C-terminal His-tag) along wit...
An Immunoglobulin Class refers to distinct classes of glycoproteins, such as IgM, IgD, IgG, IgA, and IgE, which are part of the humoral immune response. These classes are categorized based on their structural, functional, and biological characteristics. ...
New multiclass hybrid interferon polypeptides, their corresponding encoding recombinant DNA molecules and transformed hosts which produce the new interferons are described. The amino acid sequences of these hybrids include at least two different subsequences, one of which has substantial homology with a ...
Elution was concentrated to <10 mL and 1 mg of recombinant purified Tev was added to Msn5-His and incubated overnight. Tev-cleaved Msn5 was further purified in Superdex 200 Increase 10/300 GL column (Cytiva Cat. 28990944) in buffer B containing 20 mM HEPES pH 7.4, 150 mM ...