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The 5′-phosphorylated reverse complement oligo was annealed to dUssDNA 8 + 11 vector using33,35 Kunkel mutagenesis and extended to form double stranded DNA (dsDNA)36. Electrocompetent E. coli DH10B cells were used for transformations. A pool of transformants was titered to determine the...
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Smart Cities and Urban ManagementG2: Phase Change Materials for Energy StorageH: Geo-EnergyH1: Petroleum EngineeringH2: GeothermalH3: FossilI: Energy Fundamentals and ConversionI1: FuelI2: Energy and Combustion ScienceI3: Energy ChemistryJ: Thermal ManagementJ1: Heat and Mass TransferJ2: Thermodynami...