DNA was extracted using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich), analysed by Qbit and used for library preparation using one of the two protocols (kits): (1) the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) using TruSeq DNA UD Indexes 200223...
Converted DNA was then desulfonated and purified according to the manufacturer protocol, and resuspended in 60 μl H2O. We made touch-down PCR reaction (One Taq Hot start DNA polymerase, NEB) using a forward native primer and a reverse primer designed to match the converted sequence. For S...
根据第一段"It's just typical that my journey is on one of the oldest lines,as well as one of the deepest.It's the hottest on the whole tube system.(我通常上班所走的路线刚好是地铁里最老、最深的一条线。)"可知,中央线是伦敦最古老的地铁线路之一。故选A项。(2)A...
FInigpurraecti4c.alIenngpirnaecetricinagl aepnpgliinceaetiroinnsg, tahpepvliicbartaiotinosn, sthigenvailbtrhaattiohnassibgeneanl dthiaagt nhoassebdeaesnadniaogrnmoaslesdigansaal cnaonrmbeaal dsidgendaltocathnebneoardmdaeldsatmo pthlee sneotr, msoatlhseafmauplltedsieatg,nsoositshseysftaeumlt ...
开发者ID:montoyjh,项目名称:MatMethods,代码行数:34,代码来源:neb_tasks.py 示例2: _verify_inputs ▲点赞 5▼ def_verify_inputs(self):user_incar =Incar.from_file(os.path.join(os.getcwd(),"INCAR")) ref_incar =Incar.from_file(os.path.join(self["ref_dir"],"inputs","INCAR"))# perfo...
开发者ID:Motsai,项目名称:neblina-libgdx3d,代码行数:25,代码来源:Simulation.java 示例2: moveShipRight ▲点赞 3▼ importcom.badlogic.invaders.Invaders;//导入依赖的package包/类publicvoidmoveShipRight(floatdelta,floatscale){if(ship.isExploding)return;floatq0 = (float)Invaders.mInvaderInterface.getQ0...
Source File: Secp256k1.java From neb.java with GNU Lesser General Public License v3.0 6 votes public static boolean Verify(byte[] data, byte[] sign, byte[] pub) { if (data.length != 32) { throw new IllegalArgumentException("Expected 32 byte input to ECDSA verify, not...
Lok Sewa Vacancy Calendar 2081 2082 ( All Provinces) Individuals can use the calendar to participate in various public service examinations,… VIEW ALL Featured vacancies Jr Trainee Assistant wanted at a leading Commercial Bank; Freshers can APPLY ...
本文整理汇总了C#中Chummer.Skill类的典型用法代码示例。如果您正苦于以下问题:C# Skill类的具体用法?C# Skill怎么用?C# Skill使用的例子?那么恭喜您, 这里精选的类代码示例或许可以为您提供帮助。 Skill类属于Chummer命名空间,在下文中一共展示了Skill类的15个代码示例,这些例子默认根据受欢迎程度排序。您可以为喜...
v = VaspNEBJob("hello", half_kpts=True) v.setup() incar = Incar.from_file("INCAR") count = multiprocessing.cpu_count()ifcount >1: self.assertGreater(incar["NPAR"],1) kpt =Kpoints.from_file("KPOINTS") kpt_pre =Kpoints.from_file("KPOINTS.orig") ...