DNA was extracted using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich), analysed by Qbit and used for library preparation using one of the two protocols (kits): (1) the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) using TruSeq DNA UD Indexes 200223...
, the Shanghai Municipal Commission for Science and Technology (12JC1409400 to L.W.), and the State Key Laboratory of Molecular Biology. Author information Author notes These authors contributed equally: Qiyuan Yang, Ronghong Li, Qifeng Lyu, Li Hou. Authors and Affiliations State Key Laboratory ...
Versions Notes Simple Summary Osteosarcoma is the predominant form of primary bone cancer and outcomes for patients with metastatic disease have not improved for several decades. Recent genomic and pharmacological studies have implicated dysregulated histone deacetylases as druggable targets to treat several ...
Versions Notes Abstract Depending on how severe it is, malocclusion, which may involve misaligned teeth, jaws, or a combination of the two, can hurt a person’s overall facial aesthetics. The maxillary molar develops before the mandibular molar in class II malocclusion, which affects 15% of the...
Review Reports Versions Notes Abstract Integrons are genetic elements that store, express and exchange gene cassettes. These elements are characterized by containing a gene that codes for an integrase (intI), a cassette integration site (attI) and a variable region holding the cassettes. Using bioinf...
Versions Notes Abstract Stenotrophomonas rhizophilawas first discovered in soil; it is associated with the rhizosphere and capable of both protecting roots and stimulating plant growth. Therefore, it has a great potential to be used in biocontrol. The study ofS. rhizophilaphages is important for a ...
Protein Class Organism/Phylum Yield * Strain Promoter Tags Culture Type *** Notes on Protein Detection Ref. FcHyd5p II Fusarium culmorum/ Ascomycota Not reported X33 AOX1 ** No tag C-terminal 6His Flask Protein could not be readily detected without immunoassay against 6His tag [35] FcHyd3p...
Versions Notes Abstract Csa3 family transcription factors are ancillary CRISPR-associated proteins composed of N-terminal CARF domains and C-terminal winged helix-turn-helix domains. The activity of Csa3 transcription factors is thought to be controlled by cyclic oligoadenyate (cOA) second messengers ...