These species-dependent differences in the impact of PIWI loss raise the question of whether piRNAs have important functions in mammalian female germ cells. Many studies have demonstrated that small RNAs play critical roles in germ cell development in model animals11,26,27; however, the profiles ...
The authors say that they have mentioned these points in the revised manuscript in answering my third question of Section “Discussion”, while I did not find where they have mentioned. Authors’ response: We are thankful to the reviewer for appreciating our efforts. As suggested by the reviewer...
Co-founded Healthcare Exchange, a clearinghouse for matching unique opportunities with people who have the skills and time, a model for CannonDesign’s firm-wide Mentoring Exchange. Led the firm’s first Chicago–Mumbai design team; created a new model of multi-office project collaboration and st...
In short, according to the manufacturer’s protocol, 1 μg RNA was used for cDNA library construction using a NEBNext® Ul-tra™ II RNA Library Prep Kit for Illumina® (NEB #E7770). The mRNA was enriched with oligo(dT) beads, followed by two rounds of purification and random ...
GK11 was used as an out-group. Gene sequences were used for the phylogenetic analysis, which was performed using the Maximum Likelihood (ML) method, as implemented in the program PHyML 3.0. ML bootstrap supports were calculated after 100 iterations. The nucleotide substitution evolution model ...
The model has been deposited in the Protein Data Bank (6W11). Figures were generated in Pymol [38]. Table 2. Model refinement. 2.7. Electromobility Shift Assays (EMSAs) EMSA fragments were generated by PCR using Phusion® DNA Polymerase, S. solfataricus P2 genomic template DNA, and the...
This sequence of interest was generated after various sequences that have significant alignments with the sequence in question were retrieved. Approximately, twenty-one (21) sequences in conjunction with the query sequence were selected based on their E-values. This refers to the number of times ...