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(2003) reactions for each sample were performed using the Bio-Rad iQCycler Real-Time Detection System in Bio-Rad iCycler 96-well plates, where triplicate no-template controls were also used. The thermo- cycler protocol for intI1 and 16S rRNA reactions consisted of 95 1C for 15 min, ...
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For isothermal calorimetry tests, similarly, mixed liquids were added to the mixed solids in a glass vial, and the manual mixing of the pastes continued for one minute. Then, the sample vial containing the obtained paste was lowered into the micro reaction calorimeter for analysis. 2.3. ...
Virions (108 cfuampR/lane) were separated by non-reducing and reducing 4-12% Bis/Tris XT Criterion precast SDS-PAGE (Bio-Rad, Hercules, Calif., USA), and blotted onto a polyvinylidene fluoride membrane (Millipore, Madison, USA) in Tris/glycine buffer (25 mM Tris, 192 mM glycine, and ...