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Different aroA enzymes from various organisms have been divided into two classes on the basis of their intrinsic glyphosate sensitivities and their substantial sequence variations9. In general, class I aroA enzymes are natu- rally sensitive to glyphosate and generally found in Escherichia coli, ...
DNA (2 μg ) was added to room temperature thawed cells in 2 mm electroporation cuvettes, and pulsed with 2100 V and a pulse length of 1.1 ms using the square wave setting (GenePulser, BioRad). Bacteria were transferred to 10 mL THY and recovered at 37 °C. After...
gonorrhoeae and other Gram-negative organisms52, but it is the standard of care for the treatment of methicillin-resistant S. aureus and the observed PK/PD magnitude matched published work, corrobo- rating the model53,54. Levofloxacin serves as a mechanistic comparator for topoisomerase inhibitors,...