Antibodies PGT128, PGT145 and F105 were equipped C-terminally with either a biotin label or with a V5 label using sortase-directed coupling and were combined with streptavidin (SA) donor beads and anti-V5 acceptor beads. AlphaLISA assay was carried out in PBS + 0.05% Tween-20 +...
label” to provide colorings and statistics of the relationships between labels. Here the “item” column contains the name of each cell (barcode) and the label is any property of the cell (the tissue of origin, hour in a time course, celltype, etc.). You can also...
Next, we tested the ability of the bivalent and trivalent vaccine formulations to protect hamsters from challenges with the Omicron variants BA.5 and XBB.1. Six weeks after immunization, hamsters were inoculated intranasally with 105pfu of BA.5 or XBB.1 and the viral titers in the lungs and...
Solid circles on branches label the clusters strictly concordant with the host phylogenies. Open circles designate host-specific lineages without coevolutionary signal. Solid vertical lines indicate reciprocally monophyletic groups of symbionts and hosts. Dashed lines show paraphyletic symbiont clades ...
5. Guerrero-Araya, E. et al. Origin, genomic diversity and microevolution of the Clostridium difficile B1/NAP1/RT027/ST01 strain in Costa Rica, Chile, Honduras and Mexico. Microb. Genom. 6, e000355 (2020). 6. Moura, H. et al. Proteomic analysis and label-free quantification of the ...
Forcellranger, note that the-1,-2, etc. postfixes denote the first, second, etc. label in the aggregationcsvfile used as input forcellranger aggr. Default run We can now run thetoo-many-cellsalgorithm on our data. The resulting cells with assigned clusters will be printed tostdout(don’...
10 for 5 min (HK085-5 K, BioGenex); link was by biotinylated rabbit anti-goat (BA-5000, Vector) at a 1:100 dilution for 10 min with 4 + streptavidin alkaline phosphatase label for 10 min (AP605H, BioCare Medical). Staining was with warp red chromogen kit for 5 min (WR8065,...
The emergence of >300 serovars of Leptospira confounded the use of generalized bacterin, the whole cell lysate, as vaccines to control leptospirosis. Because of substantial genetic and geographic heterogeneity among circulating serovars, one vaccine s
Ward’s groups can be compared to the available set of plant labels, accepting that for hybrid samples parent selection is done with the one parent most fitting the category becoming selected (‘label assignment’). Additionally, some explanation is given about the plant names as traditionally ...