Molecular biology grade DMSO was purchased from Sigma Aldrich (St. Louis, MO, USA), Sterile PBS was from ThermoFisher Scientific (Waltham, MA, USA). 3CLpro inhibitor screening enzymatic assay kits (Catalog #79955-1) were from BPS Biosciences (San Diego, CA, USA). Amprenavir, Atazanavir su...
BrdU- injections (Sigma-Aldrich, St. Louis, MO, USA, 50 mg/ Sellner et al. Acta Neuropathologica Communications (2016) 4:102 Page 3 of 13 kg in 0.9 % NaCl, 8 h apart) over 3 days [25]. Animals were perfused 3 weeks after the last BrdU injection. Histology Histology was performed...
(St Louis, MO, USA).17 Cell culture Human ccRCC cell lines (786-O and A-498) were purchased from BioResource Collection and Research Center (Hsinchu, Taiwan) and grown in a culture medium (RPMI-1640 for 786-O cells and α-MEM for A-498 cells) containing 100 units/mL penicillin, ...
Cells were washed three times in ice-cold phosphate-buffered saline (PBS) and incubated at room temperature for 5 to 10 minutes with PBS containing 2 mg/ml lidocaine (Sigma-Aldrich, St. Louis, MO) and 0.05 mol/L ethylenediaminetetraacetic acid (Sigma-Aldrich). Cells were harvested by ...
Uridine-50-diphosphoglucuronic acid (UDPGA), tris mefenamic acid and dodecyl polyglycol ether were purchased from Sigma-Aldrich (St. Louis, MO, USA). 7-hydroxycoumarin was gained from AlfaAesar (Shanghai, China). Warfarin sodium was purchased from J&K Scientific (Beijing, China). The pooled ...
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The samples were rehydrated slowly in graded ethanol washes and then stained overnight in equal amounts of Gills solution (GHS-332; Sigma-Aldrich, St. Louis, MO) and Harris hematoxylin solution (HHS-32, Sigma-Aldrich). After several washes with water and ammonium hydroxide and with increasing...
(SGVTFQ↓GP) connected to a His6tag. The N-terminal flanking sequence is autoprocessed during expression in E. coli (BL21 DE3), whereas the C-terminal flanking sequence is removed by the treatment with PreScission protease (Millipore Sigma, St. Louis, MO). For crystallization, the authentic...