A reverse transcription reaction was performed using the miRCURY LNA™ Universal RT microRNA PCR system (Exiqon, Denmark) according to the manufacturer’s instructions. In brief, approximately a total of 100 ng of total RNA, including small RNA, were anchor-tailed with a poly(A) sequence at...
Lanes labeled with the same number display the PCR product obtained using the same primer pairs, as shown in the key: (1) 1 kb ladder (NEB); (2) ex5/in5 primers (brd2b-S 250 bp predicted product); (3) ex5/6 primers (brd2b-L 400 bp predicted product); (4) ex9/10 primer...