Leica). Fluorescence images were transformed in binary system by ImageJ to quantify the percentage of cell adhesion. In adhesion assays, bound fluorescently labeled cells were lysed and quantification was carried out using a Varioskan plate reader...
A reverse transcription reaction was performed using the miRCURY LNA™ Universal RT microRNA PCR system (Exiqon, Denmark) according to the manufacturer’s instructions. In brief, approximately a total of 100 ng of total RNA, including small RNA, were anchor-tailed with a poly(A) sequence at...