2c, left). In a subsequent 6% urea–polyacrylamide gel, the electroeluted RNAs I and II both exhibited a minor species that either matched the linear intron or the nicked 3×Flag, suggesting that I is the circular intron and II is the circular 3×Flag (circ3×Flag; Fig. 2c, right)...
We sought to identify genomic loci producing more circular than linear transcripts. The ratio of circRNA to mRNA isoforms was >1.2 for 45 genes (Fig.1d, Supplementary Fig.1, Supplementary Data3), which includedXRN2(ratio: 5),GRK3(ratio: 4) andNBAS(ratio: 1.55). The circRNAs expressed in...
aIllustration of the annotated genomic region of human circMET, different splicing forms of MET premRNA, and the validation strategy for the existence of circular MET exon 2. Convergent (black) and divergent (purple) primers were designed to amplify the linear or back-spliced products. PCR anal...
Nevertheless, circular initiatives implemented so far are incremental and limited in scope because they are oriented towards emission reduction, energy and resource efficiency and mere waste reduction which still lies in a linear thinking frame [34, 36]. Numerous scholars have highlighted that the curr...
(2021) introduced the CE in the 1970s as an alternative to the linear industrial model. The transition to CE is challenging due to assumptions, aiming to decouple resource use from economic growth. According to the authors, CE's evolution, the 10Rs hierarchy synthesis, CE roadmap, and the...
As discussed in the methodology section, the framework intends to transform a linear product supply chain to a circular product supply chain for buildings in order to capture the environmental benefits of adaptive reuse discussed in the Introduction. Step 3 of the methodology, “Defining the Building...
an SCZ-related linear isoformAS3MTd2d3[39] was also regulated by the SCZ risk loci (Fig.6b), implying a potential mechanism between the linear and circular isoform regulated by SCZ risk loci. Notably, 72.6% (114 of 157) circRNAs could be regulated by GWAS-linked circQTL SNPs located in...
A year in which we took another step forward together with you in advancing the transition from a linear to a circular economy. A year full of global political unrest, reminding us once again that unity is essential if we want to make a difference. Networks hold the greatest power. ...
To evaluate the presence of microhomology around the circular DNA junctions, we generated files that include 100 bp around the start and end of the circle (50 bp inside the circular DNA and 50 bp of linear DNA). To be able to perform this analysis, we filtered out all the circles...
Total RNA was treated with RNase R to degrade the linear RNAs and purified by using an RNeasy MinElute Cleanup Kit (Qiagen, Venlo, The Netherlands). The subsequent approach was the same as the procedure used to construct the strand-specific rRNA-depleted RNA-seq library mentioned above. All...