To confirm the predicted back splice event and circular structure of circCCNT2, primers were sequence validated for amplification of the predicted back-splice junction, and an RNase R treatment assay was used to confirm the circular structure. Sanger sequencing confirmed that our primers were speci...
circRNAs detected in this study.Y-axis: the abundance of circRNAs classified by different lengths.eThe Venn plot of the numbers of called circRNAs in NHA and U251 by RNA-seq or RNC-seq. RNC-seq identified more circRNAs due to the higher sequencing depth (see Supplementary Figure1).fScatter...
CircRNAs are mainly divided into three groups based on their components: 1) exonic circRNAs (ecircRNAs) [34]; 2) ElciRNAs or retained-intron circRNAs [35]; 3) circular intronic RNAs (ciRNAs) [36] (Figure 1). EcircRNAs, consisting of one or more exons, make up the vast majority (...
29k Accesses 1 Altmetric Explore all metrics Abstract In 2015, the United Nations Member States developed a collective blueprint for sustainability and development. The 2030 Plan includes the 17 UN-SDGs, which are an immediate call for action from all countries in the form of a global collaborat...
Mutagenic NHEJ of this DSB could generate indels inducing “3n + 1”-bp frame-shift, thus reframing GFP to the correct frame and making the cell GFP+. GFP+ cells could be measured by flow cytometry. Therefore, the percentages of GFP+ cells at 72 h post-transfection could reflect the...
The outer pit was excavated in the stiff to hard silty clay, which was retained by 1.2 m thick and 45–50 m high unpropped circular DW; the inner pit extended completely through the upper silty clays and into the clay-gravel-cobble Field instrumentation To investigate performance of ...
Consistent with the data from the microarray analysis, hsa_circ_0000326 was found to be markedly upregulated in an expanded cohort of lung adenocarcinoma tissues and adjacent tissues (N = 100) by RT-PCR (Fig. 1d). Fig. 1 Upregulation of hsa_circ_0000326 was correlated with aggressive ...
Fig. 1. Overview of the main cycles and steps carried out for the development of the MATChE CE readiness tool. 3.1. Cycle 1: theoretical development The first step in the MATChE tool development process was to carry out a literature review, to firstly ascertain important success factors, drive...
Alterations of RNA editing that affect the secondary structure of RNAs can cause human diseases. We therefore studied RNA editing in failing human hearts.
Non-coding RNAs (ncRNAs) have been shown to regulate gene expression involved in tumor progression of multiple malignancies. Our previous studies indicated that large tumor suppressor kinase 1 (LATS1), a core part of Hippo signaling pathway, functions as