acc=GSE97332 ,的差异分析,网页工具或者代码都可以, 做出一个热图即可,详见:所以GSE号也可以弄错吗。 (A) The heat map showed the top 10 most increased and decreased circRNAs in HCC tissues as compared to that in the matched nontumor tissues
CircRNA, as a type of endogenous non-coding RNA (ncRNA), was first found in the virus in 1976. But it was considered as one of the non-function by-products from splicing errors in a long time [1,2,3], thus, it was thought unlikely to play important role in biological processes. Wi...
g., the related products of Arraystar (https://www.arraystar. com/) and Cofactor Genomics (https://cofactorgenomics. com/) [46]. In addition, circRNA-associated bioinformat- ics tools including circRNA_finder, find_circ, CIRCex- plorer, CIRI and MapSplice, ...
The circRNA dataset (GSE131969) included data from three ESCC tissue and three adjacent noncancerous tissue samples. The samples were tested against GPL19978 Agilent-069978 Arraystar Human CircRNA microarray V1 data. The miRNA dataset (GSE97051) included data from seven ESCC tissue and seven non...
Linear RNAs were removed from total RNAs using RNase R to enrich circular RNAs, which were then amplified and transcribed into fluorescent cRNA with a random priming technique (Arraystar Super RNA Labeling Kit; Arraystar). Labeled cRNAs were purified using the RNeasy Mini Kit (QIAGEN), and ...
Arraystar Proprietary Algorithms based on the public databases TargetScan and miRanda, and the highest ranking candidate miRNAs were selected. At least one miRNA-binding site identified by the Arraystar Proprietary Algorithms was confirmed using the CircInteractome database (Supplementary Figure 6A). To ...
数据集(3个巨噬细胞对照样本,3个氧化型低密度脂蛋白(OxLDL)处理的巨噬细胞样本)和GPL19978(Agilent-069978 Arraystar Human CircRNA microarray V1)平台注释文件,通过编写perl脚本用平台注释文件对GSE107522表达矩阵的探针进行注释获得circRNA表达矩阵,接着使用AgilentcircRNA注释文件将circRNA的ID环化为circBase格式的ID。