The chapter presents a discussion on plasmid containing the λ P L promoter and ci857 repressor. Cells harboring a plasmid containing P L also need to contain the λ cI repressor to turn off the promoter. One approach to provide the λ cI repressor is to use a λ lysogen containing the ...
These results strongly suggest that the thermosensitive CI857 repressor, encoded by the expression vector, competes with CI Ind− molecules for binding to the prophage operator region, allowing for expression of lytic genes from the integrated Ind− viral genome upon temperature up-shift. Transcript...
The system can still stably suppress gene expression of E at 37 ℃, when the temperature exceeds the highest temperature still repressor normally induced bacterial lysis, facilitates rapid growth of bacteria and the surface of the important epitopes holding clusters, and can conventional manner to ...
F., and Delorbe, W. J. (1987), Bio/Technology 5 , 600–603.George, H. L., R. J. Watson, D. F. Harbrecht, and W. J. DeLorbe. 1987. A bacteriophage cI857 cassette controls PL expression vectors at physiologic temperature. Bio/Technology 5:600-603....
cI857 repressorEscherichia colilysis gene Epromoter pLSalmonella enteritidisAims To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo-inducible lambda cI857-pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis...