p I, isoelectric pointAbsorption spectra of aqueous solutions of Rose Bengal—(α-chymotrypsin) and Rose Bengal—(α- chymotrypsinogen-A) measured at different pHs showed that both proteins formed the same complexes. The binding constants for the formation of RB/CHT and RB/CHTGA complexes were ...
The zymogen with potential ATEEase activity has a basic isoelectric point, can be activated by , and can hydrolyse and ATEE but not ATAME. Its molecular weight is about 26,500 and the NH2-terminal sequence indicates clearly that it belongs to the chymotrypsinogen family, but that it is not ...
The progressive transition of CTG-A () to CTG-X (), a previously described biologically active carbamylated derivative (1–3), is demonstrated by isoelectric focusing in polyacrylamide gels. This technique allowed the visualization of 12 carbamylated protein species, whose isoelectric point could be...
A homogeneous preparation of chymotrypsinogen has been obtained by the fractional salting out of an acid extract of porcine pancreas and subsequent chromatography on a column of CM-cellulose. The isoelectric point of the enzyme has been found to be pH 8.8 and the optimum value of the pH for ...
Trypsinogen 1 and chymotrypsinogen can only be separated at this pH, which is close to their isoelectric point. Each of the three purified proenzymes shows a single band on polyacrylamide gel disc electrophoresis. 4. 4.|The amino acid composition of rat chymotrypsinogen resembles those of beef ...