During mitosis in eukaryotic cells, mechanical forces generated by the mitotic spindle pull the sister chromatids into the nascent daughter cells. How do mitotic chromosomes achieve the necessary mechanical stiffness and stability to maintain their integ
Subsequently, poleward forces exerted by kinetochore-bound microtubules pull apart the 16 sets of sister kinetochores and their associated pericentric DNA (Yeh et al., 2008). Chromosomes are postulated to remain in this bioriented configuration until the onset of anaphase (Gardner et al., 2005, ...
By Mazia's time it had come to refer—as it still does today—to the phase of mitosis when sister chromatids are moving apart from one another toward opposite sides of the cell. The onset of anaphase is one of the most abrupt events of mitosis, making it cytologically useful as a ...
At this point, once the cell enters metaphase, the homologous chromosomes will begin lining up in pairs along the metaphase plate, guided and controlled by the spindles, which emerge from the centromere. In the final stage of meiosis I, anaphase, the homologous chromosomes are pulled apart into...
This generated strains in which small fluorescent dots were present on chromosomes at the positions of the TetO arrays. Because the mitotic spindle moves continuously during mitosis, determining the movement of chromosomes relative to the spindle requires knowledge of the spindle axis. In previous work...
This led to the view of DNA replication and DSB formation as independent events, which are initiated by a common mechanism. Indeed, the cyclin-dependent kinase (CDK) that promotes S phase also promotes Spo11 activity (Henderson et al., 2006, Smith et al., 2001). Whether regulators of DNA...