During cell division, chromosome congression to the spindle center, their orientation along the spindle long axis and alignment at the metaphase plate depend on interactions between spindle microtubules and kinetochores, and are pre-requisite for chromos
we projected the positions of all attached KMT ends on to the plane of cell division. There were 6 to 50 KMTs attaching to each of the 12 chromosomes per pole-facing side (Fig. 1j). Despite the larger kinetochore region, this is surprisingly close to the number...
Therefore, we developed a novel method to analyse the EBP-2 velocity within the spindle based on spatial–temporal correlation (see also Methods). We analysed four different regions: within the spindle, at chromosomes, and within a central (inner astral) and a peripheral (outer astral) region ...
During metaphase, the cell's chromosomes align themselves in the middle of the cell through a type of cellular "tug of war." The chromosomes, which have been replicated and remain joined at a central point calledthe centromere, are called sister chromatids. In which phase of the cell cycle ...
In mitotic cells (Fig. 3a; Supplementary Movie 1), 10 holocentric chromosomes align along the metaphase axis and each is attached to the spindle at various sites in a bipolar manner (amphitelic attachment). The kinetochores can be visualized all along the chromosome as parallel axes of CENH3...