Implication of Chromosome Structure and Replication on Hazard of Tritiated Thymidine and the Interpretation of Data on Cell Proliferationbiology and medicineamino acidschromosomesgeneticsmanmitosisnucleic acidsradioautographytissuestritiumLABELLING of chromosomal deoxyribonucleic acid (DNA) with tritium-labelled ...
Ultimately, the actin cables formed a structure resembling a shell that encapsulated the clustered chromosomes (Fig. 4a). Importantly, the actin cables were also detectable in fixed oocytes stained with phalloidin and are thus not an artefact caused by the EGFP-UtrCH probe (Extended Data Fig. ...
Local compartmentalization of chromatin is consistent both with a multi-loop model[20]of interphase chromosome structure (akin to the radial-loop model of the metaphase chromosome) and with the random-walk or giant-loop model[23], although these two models make different predictions about the ...
Bionano Genomics data were generated from the CHM13 genome13. Long DNA molecules labelled with Bionano’s Direct Labelling Enzyme were collected on a Bionano Saphyr Instrument to a coverage of 130×. The molecules were assembled with the Bionano assembly pipeline Solve (v.3.4), using the non...
Fig. 4.Scaffold region of a histone-depleted human mitotic chromosome. Chromosomes isolated from HeLa cells were depleted of histones by treatment with 2 M NaCl and prepared by Kleinschmidt spreading[460]. Note the open, network-like structure of the scaffold in the chromatid on the left. ...
These observations show that in HeLa and TG cells, a particular structure present at the chromosome periphery in the form of PCM is persistent and ubiquitous. In addition, we showed by immunolabelling that the PCM is the specific site of accumulation of nucleolar antigens during mitosis. These ...
(C) Projections of deconvolved image stacks showing the asymmetric labelling of Bub1 on the kinetochore of mono-orientated chromosomes. (D) Bar graph quantifying inter-kinetochore distance at metaphase chromosomes. Values represent the mean and s.e.m. derived from at least 20 kinetochores in at ...
Molecular karyotyping was performed on the patient, his parents, maternal grandmother and maternal aunt using Human Genome CGH Microarray Kit G3 180 (Agilent Technologies, Palo Alto, USA) with ~13Kb overall median probe spacing. Labelling and hybridization were per- formed following the protocols ...
although less markedly than the F-element. Interestingly, the left arm of chromosome X inDpseparates in the second component while the right arm clusters closer to the other chromosomes. This is in agreement with the hypothesis that the right arm ofDpX is a later addition [15]. The left ar...
At the single-cell level, the torus is strikingly heterogeneous, with blob-like Mbp-size domains that undergo major dynamic rearrangements, splitting and merging at a minute timescale. Our data show a domain organization underlying the chromosome structure of E. coli, where MatP proteins induce ...