Implication of Chromosome Structure and Replication on Hazard of Tritiated Thymidine and the Interpretation of Data on Cell Proliferationbiology and medicineamino acidschromosomesgeneticsmanmitosisnucleic acidsradioautographytissuestritiumLABELLING of chromosomal deoxyribonucleic acid (DNA) with tritium-labelled ...
Probe preparation. Single-stranded cDNA or EST is used for labelling and fluorescent dye is covalently attached to the DNA. 3. Hybridization. The chromosome or the DNA attached to the glass slide is denatured with formaldehyde to render it single stranded. The labelled probes are flooded on the...
Ultimately, the actin cables formed a structure resembling a shell that encapsulated the clustered chromosomes (Fig. 4a). Importantly, the actin cables were also detectable in fixed oocytes stained with phalloidin and are thus not an artefact caused by the EGFP-UtrCH probe (Extended Data Fig. ...
and Pierce SK (2014) Fish labelling reveals a horizontally transferred algal (Vaucheria litorea) nuclear gene on a sea slug (Elysia chlorotica) chromosome... JA Schwartz,NE Curtis,SK Pierce - 《Biological Bulletin》 被引量: 0发表: 2014年 Horizontal gene transfer of the algal nuclear gene psbO...
Reveals Differences in Chromosome Structure International Journal of Molecular Sciences 21(21) Hayes, H. 1995: Chromosome painting with human chromosome-specific DNA libraries reveals the extent and distribution of conserved segments in bovine chromosomes Cytogenetics and Cell Genetics 71(2): 168-174 ...
There are 1287 MSY specific STS markers encompassing the human Y chromosome with an average spacing of 14 Kb spanning over 1698 loci [17]. Deletion mapping based on the STSs has advanced our understanding of the Y chromosome structure and functions. Previous studies were mostly conducted on ...
(C) Projections of deconvolved image stacks showing the asymmetric labelling of Bub1 on the kinetochore of mono-orientated chromosomes. (D) Bar graph quantifying inter-kinetochore distance at metaphase chromosomes. Values represent the mean and s.e.m. derived from at least 20 kinetochores in at ...
With these labelling strategies, it has been found that Drosophila metaphase chromosomes contains fine filaments of ~70 nm17. More recently, 3D STORM combined with oligonucleotide probes has distinguished the structures among active, inactive, and repressed chromatin19. In addition, telomeric chromatin...
Broad axial localization of condensin and Topo IIα have also been observed using EM with immunogold labelling7,8. In contrast, micromechanical force measurements suggest that chromosomes have no rigid scaffold9. Due to these discrepancies, the structure of the chromosome scaffold still remains ...
2. Clean up with ethanol precipitation by array processing laboratory. 3. Process samples as per Agilent SureTag complete DNA labelling kit—Agilent Oligonucleotide Array-based CGH for Genomic DNA Analysis Enzymatic labelling for Blood, Cells or Tissues 4. Scan using Agilent C Scanner using Agilent...