S. pneumoniaestrain R36A, an avirulent, nonencapsulated strain, was grown to mid-log phase in Todd-Hewitt broth + 1% yeast extract (Becton Dickinson, Sparks, MD) and stored at − 80 °C. For immunization, stock was cultured in a candle jar for 18 h at 37 °C on blood a...
Genetic Counseling in a Patient With XXY/XXXY/XY Mosaic Klinefelter's Syndrome: Estimate of Sex Chromosome Aberrations in Sperm Before Intracytoplasmic Spe... High resolution karyotype was performed in 13 retinoblastoma patients. A mosaic pattern for del(13)(q14.1; q14.3) was found in a girl ...
For early female (female before laying eggs) collection, we collected plants 18 days after nematode infestation and picked out early females in root knots that had not produced egg masses with tweezers. For female collection, we removed the egg masses at the root knots and manually picked out ...
Heterochromatin maintains genome integrity and function, and is organised into distinct nuclear domains. Some of these domains are proposed to form by phase separation through the accumulation of HP1ɑ. Mouse heterochromatin contains noncoding major sate
Instead of cleaving into two daughter cells, however, the excess half complement of chromosomes are shunted into a rudimentary structure called the first polar body (PB1 in Figure 1.14) in a clearly asymmetric division. There is no intervening S-phase before entry into meiosis II, and oocytes...
It is proposed that the number of structural effectors varies discontinuously both just before mitosis through an "all-or-none event" and during the mitotic process itself. The effector persists from mitosis to mitosis, but is not active in G2 due to the polymerization of soluble monomers. The...
Comparison of mouse and human colon tumors with regard to phase I and phase II drug-metabolizing enzyme systems. Since human colorectal tumors are insensitive to most chemotherapeutic agents, there is a need for the discovery of new drugs that would show activity agai... L Massaad,I De Wazier...
On the other hand, in case of DAPI staining, the macerated root tips of octoploid plants were soaked in 60% acetic acid for 5 min before staining. Clear digital images of F. vesca and F.×ananassa were obtained using light and fluorescent microscopy. Their respective 14 and 56 ...
[8]. The genome ofLiriodendron chinensehas evolved through an ancient WGD event and recent repetitive sequence bursts, leading to specific patterns of floral color and petal evolution [9]. An ancient WGD event that occurred before the radiation of most Ericaceae plants contributed to the genomic ...
before the scaffolding. The use of the independent DNA sources reduced contamination from DNA sequences of organelle genomes and microorganisms while preserving nuclear-integrated organellar sequences. In addition, a novel approach for the discovery of genome structural variation based on quantitative ...