The underlying principle is – as the sample solution makes contact with the second solid or liquid phase, the solutes will start interacting with the other phases. Due to different adsorption rates, ion-exchanges, partitioning or sizes, the interaction will vary, and that’s what will enable ...
The principle of separation is thus by reversible exchange of ions between the target ions present in the sample solution to the ions present on ion exchangers. In this process, two types of exchangers i.e., cationic and anionic exchangers can be used. Cationic exchangerspossess negatively charge...
Chromatography is a very efficient and reliable technique of separation of compounds or mixtures by making them pass via a stationary phase at different rates. The principle works on differential affinities of the compounds. The stationary phase may vary according to the compound to be separated ...
The principle of paper chromatography is based on the varying degree of interaction between the constituents and the stationary phase. It comprises of two types, namely, paper adsorption and paperpartition chromatography[35]. The former deals with the ability of the compounds present in the mixture...
Principle of Column Chromatography In column chromatography the stationary phase is packed into a glass or metal column. The mixture of analytes is then applied and the mobile phase, commonly referred to as the eluent, is passed through the column either by use of a pumping system or applied ...
This current indisputable status of chromatography is reflected by the fact that majority of the present techniques is based on the principle of chromatography. This review focusses on the various types of chromatography, their working principles and applications. 展开 ...
■General principle for column chromatography Gel selection Make target protein elute out column earlier Bead size Finer bead has better resolution, slower flow rate Column size Use larger column size but consider practical need Flow rate Fast flow reduces resolution, slow Sample volume Apply 1% of...
Although the principle above works even in simple techniques such as paper chromatography, modern chromatographic equipment today employ ananalytical columnwhere the actual separation process takes place. The column is usually made of glass or metal tube that is capable of withstanding the range of pre...
Briefly describe the principle of thin layer chromatography. In what way are normal-phase partition chromatography and adsorption chromatography similar? Explain the difference between chromatography and solvent extraction. Explain how thin-layer chromatography (TLC) works. ...
Among these, magnetic solid-phase extraction (MSPE) is a rapid and efficient sample-pretreatment technology that is based on the similar solid-phase-extraction (SPE) principle, which mainly enriches target analytes by exploiting their interactions with functional groups on the surfaces of magnetic ...