Current methods to identify sites of RNA interaction with the genome are limited to the study of a single RNA at a time. Here we describe a protocol for ChAR-seq, a strategy to identify all chromatin-associated RNAs and map their DNA contacts genome-wide. In ChAR-seq, proximity ligation ...
RNAi pathways have evolved as important modulators of gene expression that act in the cytoplasm by degrading RNA target molecules via the activity of short (21-30nt) RNAs1-6 RNAi components have been reported to play a role in the nucleus as they are involved in epigenetic regulation and ...
Mammalian cells in different states of cytodifferentiation exhibited different RNA-synthesizing and processing patterns that could be used as markers for phenotypic variability. Inherent in these patterns was an RNA class which was differentially extracted from the cellular homogenate by elevating the temper...
We found that a cluster of long noncoding RNAs, ELEANORS, is specifically expressed in ER (estrogen receptor)-positive recurrent breast cancer cells, forms a molecular condensate (RNA cloud) in the nucleus, and activates the ESR1 gene that encodes ER. ELEANORS define a large chromatin domain,...
We present an overview of the role of specialized RNA interference machineries and their associated noncoding RNAs in the control of DNA elimination. Finally, we discuss how RNA-dependent modification and/or remodeling of chromatin may ... S Duharcourt,M Betermier - 《Microbiology Spectrum》 被引...
To unify the structural and enzymatic aspects of SAF-A function, we speculate that SAF-A/RNA interactions will drive the formation of local chromatin domains or micro-bodies (Brackley et al., 2016), while its role in regulating large-scale chromatin structures will partition the genome into fu...
elegans. Using an RNA interference (RNAi)-based approach, we identified UBXN-3 (ubiquitin regulatory X (UBX) domain-containing protein in nematodes 3) as a crucial factor that coordinates CDC-48-dependent DNA replication events. Embryos with reduced protein levels of both UBXN-3 and CDC-48 ...
Characterization of the RNA content of chromatin. To uncover the functional role of ncRNA in chromatin organization, we deep sequenced chromatin-associated RNAs (CARs) from human fibroblast (HF) cells. ... Mondal,Tanmoy,Rasmussen,... - 《Genome Research》 被引量: 204发表: 2010年 Perspectives ...
The RNA chaperone protein, CIRP, plays a critical role in upregulating the inflammatory cascade when released from cells as eCIRP in acute inflammatory conditions, including sepsis. Elevated plasma levels of eCIRP have been independently correlated with worse prognosis in human sepsis [20,125]. Intrac...
insertional mutagenesis, microsatellite instability, transgenic plants, RNA interference, transfer DNA, AtMSH2 gene, gene silencing, molecular sequence data, ... JM Leonard,SR Bollmann,JB Hays - 《Plant Physiology》 被引量: 147发表: 2003年 ...