In eukaryotes, the deoxyribonucleic acid (DNA) molecule is associated with nuclear proteins called histones to form chromatin. This structure is not only essential to compact DNA to fit into the cell nucleus but also contributes to the regulation of DNA- dependent molecular mechanisms. Epigenetic ...
First, it is probable that in many ukaryotic cells, the genome is repressed at any one time. Therefore, biochemical or physical studies of total chromatin refer primarily to the genome in its inactive state so that features characteristic of active chromatin may be obscured. Furthermore, the ...
Chromatin structure, and in particular highly-condensed chromatin, is generally inhibitory to transcription, replication andDNA repair. Therefore, all eukaryotesfaceanother challenge, namely, to counteract the inhibitory effects of chromatin in order to accomplish these essentialcellular processes[1,2]. An...
In eukaryotes, DNA is tightly wound into a complex called chromatin. Thanks to the process of chromatin remodeling, this complex can be "opened" so that specific genes are expressed. AaAaAa Chromatin If theDNAstrand in a single humancellwere stretched out, it would measure several meters in ...
This animation starts by depicting both the protein and DNA components of the nucleosome, which is the fundamental unit of chromatin structure in eukaryotes. The eight histone proteins are shown in green, and the DNA helix in blue and white. The canonical nucleosome core particle consists of 147...
While these bacteria lack the chromatin structure found in eukaryotes that would ultimately be the namesake of the technique, by identifying specific RNA polymerase binding sites within the bacterial genome, Gilmour and Lis (1984) demonstrated that protein-DNA interactions could be fixed by covalent ...
Trifonov EN: Nucleosome repeat lengths and columnar chromatin structure. J Biomol Struct Dyn 2015:1-6. Based on the quantitative analysis of nucleosome repeat length (NRL) distributions, the author proposed a dominant role of columnar chromatin structure (Figure 2c) in all eukaryotes....
The structural organisation of chromatin in eukaryotes plays an important role in a number of biological processes. Our results provide a comprehensive insight into the nuclear topography of human peripheral blood granulocytes, mainly neutrophils. The nuclei of granulocytes are characterised by a segmented...
We have shown previously that several functionally linked genes, encoding proteins involved in chromatin-structure dynamics, which are conserved in S. pombe and other crown-group eukaryotes, have been lost as a group in S. cerevisiae [ 43].How...
Cas9 DNA targeting is inherently sequence specific but not temporally controlled. Here, authors spatiotemporally couple Cas9 activity to target site transcription in eukaryotes and exploit this to preferentially edit the more highly transcribed of two alleles that harbor identical Cas9 targets. ...