(2001) Differential chromatin association and nucleosome binding of the maize HMGA, HMGB, and SSRP1 proteins. Biochemistry, 40, 7860-7867.Lichota,J. and Grasser,K.D. (2001) Differential chromatin association and nucleosome binding of the maize HMGA, HMGB and SSRP1 proteins. Biochemistry, 40, ...
The basic repeating structural (and functional) unit of chromatin is the nucleosome, which contains eight histone proteins and about 146 base pairs of DNA (Van Holde, 1988; Wolffe, 1999). The observation by electron microscopists that chromatin appeared similar to beads on a string provided an...
Remodeling by ISW2 but not SWI/SNF requires DNA torsional strain near the site of translocation, which may work in conjunction with conformational changes of ISW2 to promote nucleosome movement on DNA. The difference in step size of nucleosome movement by SWI/SNF and ISW2 demonstrates how SWI/...
The relationship between chromatin organization and gene regulation remains unclear. While disruption of chromatin domains and domain boundaries can lead to misexpression of developmental genes, acute depletion of regulators of genome organization has a
The DNA and histone proteins provide the first level of compaction for DNA inside the nucleus. The basic unit of structure of chromatin is the nucleosome. A nucleosome is formed when DNA is wrapped around histones (the protein core) to form a “bead-like” structure. This bead-like structure...
We have recently reported that certain core histone-DNA contacts are altered in nucleosomes during chromatin unfolding (Usachenko, S. I., Gavin I. M., and Bavykin, S. G. (1996) J. Biol. Chem. 271, 3831–3836). In this work, we demonstrate that these alterations are caused by a ...
The basic unit of chromatin is the nucleosome, in which 147 bp of duplex DNA are wrapped around a histone octamer containing two copies of each of the four core histones: H2A, H2B, H3 and H43. The higher-order organization of nucleosome core particles is controlled by the association of...
Moreover, although the EcoRI-solubilized α-oligonucleosomes contain one EcoRI site in every 172-bp α-DNA repeat, they are completely resistant to redigestion with EcoRI. This striking difference between the EcoRI-accessible EcoRI sites flanking an EcoRI-solubilized α-oligonucleosome and ...
The nucleosome is further folded to produce a chromatin fiber. Chromatin fibers are coiled and condensed to form chromosomes. Chromatin makes it possible for many cell processes to occur includingDNA replication,transcription, DNA repair,genetic recombination, and cell division. ...
Here, we use Micro-C-XL, a high-throughput chromosome conformation capture (Hi-C)-based technology that involves micrococcal nuclease (instead of restriction enzymes) and long cross-linkers, to dissect single nucleosome-resolution chromatin organization in Arabidopsis. Insulation analysis reveals more ...