1、 激活型光敏蛋白: (1) ChR2(H134R):视紫红质通道蛋白2(Channelrhodopsin-2,ChR2)的一种突变体,属于光控阳离子通道,在蓝光(更佳激活波长450nm)的激发下打开阳离子通道,使胞外阳离子内流。与ChR2相比,ChR2(H134R)的光敏感性较高,通道关闭速度较慢,因此增加了光电信号,但这也使得它的时间精度不如ChR2。
将CAG-LSL-chR2(H134R)-tdTomato-WPRE-pA插入到小鼠Rosa26基因中。 应用领域:光遗传性研究 *使用本品系发表的文献需注明: R26-CAG-LSL-ChR2-H134R-tdTomato mice (Cat. NO. NM-KI-190113) were purchased from Shanghai Model Organisms Center, Inc.. ...
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA catalog : 65454 more info or order: Addgene product webpage citations: 1 Reference Madisen L, Garner A, Shimaoka D, Chuong A, Klapoetke N, Li L,et al. Transgenic mice for intersectional targeting of neural sensors and effectors with...
Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation growth temp : Stbl2 E coli from Invitrogen Grow at 30 degrees in 2xYT media origin : 30 pi : alt_names ChR2 cloning clone_methodRestriction Enzyme cloning_site_3Spe1 ...
(1) ChR2(H134R):视紫红质通道蛋白2(Channelrhodopsin-2,ChR2)的一种突变体,属于光控阳离子通道,在蓝光(更佳激活波长450nm)的激发下打开阳离子通道,使胞外阳离子内流。与ChR2相比,ChR2(H134R)的光敏感性较高,通道关闭速度较慢,因此增加了光电信号,但这也使得它的时间精度不如ChR2。