Gubelmann C, Gattiker A, Massouras A, Hens K, David F, Decouttere F, Rougemont J, Deplancke B (2011) GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR. Database (Oxford) 2011:bar040 19. Lefever S, Vandesompele J, Speleman F, Pattyn F (2008...
W. 1992. Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Molecular Ecology 1:233–240. CAS Google Scholar Delwiche, C. F, M. Kuhsel, and J. D. Palmer. 1995. Phylogenetic analysis of tufA sequences indicates a cyanobacterial origin of all ...
Hot start enzymes enhance an assay’s precision, sensitivity, and reliability by inhibiting the activity of the DNA polymerase during the initial PCR setup. This is particularly important in high-throughput systems where reactions are prepared in bulk and mul...
6. The products were purified with GeneMatrix PCR/DNA Clean-Up DNA Purification Kit (EurX) according to the manufacturer’s protocol. Sanger sequencing was performed with BrightDye Cycle Sequencing kit (Nimagen), using 40 ng of template DNA, 1.5 pmol of primer and 1 µl of kit and 1.5 ...
A fragment of 740 to 752 bp that contained the most highly variable area (positions 408 to 420) was amplified and sequenced with these universal primers for 47 strains. The variability of this sequence allowed identification of all strains and correlated well with results of DNA-DNA relatedness...
RTs catalyze the synthesis of a DNA copy (cDNA) of the target RNA molecules using a reverse transcription primer, dNTPs, and Mg2+ or Mn2+ as a cofactor. Scientists have since adapted these enzymes for use in a variety of in vitro applications including real-time and endpoint RT-PCR, ...
Learn how to determine the correct PCR cycle number for RNA-Seq library preparation based on sample properties and optimized workflows.
Escherichia coli (E. coli) is a bacteria that is widely employed in many industries for the production of high interest bio-products such as recombinant proteins. Nevertheless, the use of E. coli for recombinant protein production may entail some disadva