The NSAF was then calculated by normalizing the SAF value to the total SAF values of all the identified proteins within each sample. This approach allows for the accurate assessment of protein abundance across different samples. Immunocytochemistry Immortalized astrocytes were grown in 8-well cell ...
LDL-cholesterol concentrations showed some evidence of a non-normal distribution. Therefore, all regression analyses were done on natural log-transformed LDL-cholesterol concentrations, which showed a near normal distribution. We used linear regression analysis with an additive model adjusted for age, ...
Ratio of triglycerides to HDL cholesterol is an indicator of LDL particle size in patients with type 2 diabetes and normal HDL cholesterol levels. Diabetes Care 2000;23:1679-85.Hanak V, Munoz J, Teague J, et al. Accuracy of the triglycerides to high-density lipoprote...
A P value of <0.05 was considered statistically significant, and all statistically significant values are shown. Study approval Blood samples were obtained locally from healthy donors, and mevalonate kinase deficient patients were recruited from Royal Free Hospital NHS Foundation Trust, London, UK or ...
PopulationFluorescence intensity in plasma membrane pixels (relative values)Recovery (%) Control 100 ± 3.1 50% cholesterol depletion 33.5 ± 1.5 60 min recovery 75.1 ± 3.5 224 50% cholesterol depletion + simvastatin 25.7 ± 2.1 60 min recovery + simvastatin 61.5 ± 2.8 239 Jurkat T cells ...
Candidate metabolites were annotated using accurate m/z values, fragmentation patterns, retention times, and the METLIN database (https://metlin.scripps.edu/). MS data pre-processing The UPLC-MS raw data were acquired using MassLynx software version 4.1 (Waters, Manchester, UK) and converted in...
These two domains are related by a rigid body rotation of ~215° about the membrane normal. Approximate positions where L1 and L7 attach to TM helices (TM1 and TM2 for L1; TM7 and TM8 for L7) are indicated by circles. (D) Left: superposition of cScap and cScapD435V/cInsig-1 as ...
(GK5), a skin-specific kinase, regulates SREBP2 processing and controls cholesterol homeostasis in sebocytes, terminally differentiated epithelial cells in sebaceous glands that are required for normal hair follicle differentiation and cycling13. Hence, different tissues are impacted by dysregulated ...
Normal rabbit IgG (for ChIP 10μl/10μg of chromatin) Cell Signaling Cat#2729S;RRID:AB_1031062 Rabbit monoclonal anti-phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (clone D27F4)(for WB 1:1000; for IF 1:200) Cell signalling Cat#8828; RRID:AB_2631089 EnVision+ System- HRP Labelled...
Graph shows PLA values normalized to the abundance of Abca1 and Lxrβ. (Q) PLA of the Abca12 and Lxrβ pair in RAW 264.7 cells with or without Abca12 knockdown. Quantitation of PLA assay (right panels in O–Q) is described in the Experimental Procedures. Means ± SEM of PLA signal ...