Growth and eGFP Production of CHO-K1 Suspension Cells Cultivated From Single Cell to Laboratory ScaleScaling down bioproduction processes has become a major driving force for more accelerated and efficient process development over the last decades. Especially expensive and time-consuming processes like ...
CHO-K1 人类角质生长液说明书 TDS-ES-072-A-07 Material Provided Cells:2 x 1 mL frozen aliquots (ES-072-AV)Format: ~2.5 x 106 cells / mL in freezing medium Product Information Cellular Background:CHO-K1 Cell Line Development: Our proprietary bicistronic expression plasmid containing the se...
CHO-K1 (suspension) Chinese Hamster Ovary Cell K1 Subclone Line Boiling point Melting point Density MSDS Formula Use,CHO-K1 (suspension) Chinese Hamster Ovary Cell K1 Subclone Line 融点、価格、蒸気圧、沸点、毒性、比重、沸点、密度、分子式、分子量、物理的
Synonyms:CHO-K1 (suspension) Chinese Hamster Ovary Cell K1 Subclone Line CAS: MF: MW:0 EINECS: Mol File:Mol File Information Error Report Your Email: Browse by NationalityCHO-K1 (suspension) Chinese Hamster Ovary Cell K1 Subclone LineSuppliersChina suppliers ...
在不同的细胞系中表现不同,CHO细胞培养中添加44uM-1.1mM的EGCG提高Qp 20%50,CHO-K1细胞培养中初始接种后添加8uM的EGCG(E4143, Sigma-Aldrich)提高Qp 50%52。与在CHO DG44中不同的是,在CHO-K1中第0天添加比第4天添加表达量更高。由于EGCG的安...
RemarksMedium does not support wild type DG44 cells Culture EnvironmentCO2 Culture TypeSuspension Cell Culture Unit SizeEach 內容物與存放 1 x 10 kg, Storage 2-8°C Dark and Dry 圖表 Growth Curves and rlgG Production (Clone 2) Fed-Batch Culture Using CD OptiCHO™ as Base Medium ...
The CHOSOURCE™ Glutamine Synthetase (GS) knockout (KO) cell line is a well-established, cGMP-manufactured CHO-K1-derived suspension cell line, specifically engineered by knocking-out the glutamine synthetase gene to provide a robust industry standard selection system. The GS KO selection system ...
•使用 CHO-S、CHO-DG44、CHO-GS 和 CHO-K1 细胞系测试• 支持在 1 个体积/天 (VVD) 的连续灌注情况下超过 100 万细胞/mL 的高细胞密度• 经验证,在 1 VVD 的连续灌注情况下,生产率高达 1.7 g/L/天灵活可使用多种浓度的 HIP CHO 培养基来支持一种产品种子培养和生产。培养基的灵活配制便于动态...
5.Lee N, Shin J, Park JH, Lee GM, Cho S, Cho BK. Targeted Gene Deletion Using DNA-Free RNA-Guided Cas9 Nuclease Accelerates Adaptation of CHO Cells to Suspension Culture. ACS Synth Biol. 2016 Nov 18;5(11):1211-1219. doi: 10.1021/acssynbio.5b00249. Epub 2016 Feb 18. PMID: 2685453...
Cells convert pyruvate to lactate under anaerobic conditions or when energy demands exceed the capacity of mitochondrial oxidative phosphorylation, allowing glycolysis to continue producing ATP and NAD. References Reinhart, D., et al., Bioprocessing of Recombinant CHO‐K1, CHO‐DG44, and CHO...