the ease of collection of blood samples and the availability of technology to perform these tests make these ideal for screening large numbers of samples (particularly suited to screen livestock).6The standards
the ease of collection of blood samples and the availability of technology to perform these tests make these ideal for screening large numbers of samples (particularly suited to screen livestock).6The standardserologic testfor chlamydial antibodies is the CFT. The modified direct CFT can be used wi...
Surface-exposed PS also acts as a ligand during apoptotic clearance and blood cell clotting. Moreover, PS externalization promotes HIV uptake21. However, in these latter cases, PS exposure occurs via activated human scramblases. Exofacial PS has also been found to have a role during host-cell...
We used our full sample size of 579 when indeterminate results from the ELISA and LFA serum assays were included. With indeterminate results omitted, the sample size dropped to 569. Models that include LFA blood tests have a sample size of 297. This sample size drops to 289 for analyses whe...
trachomatis cHSP60 ELISA; MEDAC Infertile: combined results from MEDAC MOMP and MEDAC cHSP60 to predict infertile/chronic sequelae outcomes from C. trachomatis infections as per the manufacturer's recommendations; OR: odds ratio; NAAT: nucleic acid amplification test. Competing interests The authors...
Results The overall prevalence of Chlamydial infection in unexplained infertility cases as detected by both ELISA and PCR was 40 % (20/50). The prevalence of current Chlamydial genital infection as detected by real-time PCR was only 6.0 % (3/50); two of which were also IgM positive. Preval...
Genomic DNA was isolated from cells using the DNeasy Blood ®& Tissue Kit (Qiagen). RNA was isolated from an equivalent number of cells using the Direct-zol RNA Kit ™(Zymo). DNase treatment was performed to remove residual DNA. A total of 1 μg of RNA was reverse...
A purified native and recombinant 10-kD heat shock protein (HSP) has been found to be a strong stimulator of peripheral blood T-cell proliferation in Mycobacterium leprae (130). Similar strong proliferative responses of PBMC to the 10-kD HSP has been shown in Mycobacterium tuberculosis (131)....
were compared with the new antigens. It was found that all new antigens permit better discrimination between sera from patients with clinically relevant infections withChlamydia trachomatiscompared with sera from seropositive, but clinically unremarkable blood donors. The results are presented in Table ...
For example, incubation of a polypeptide of the invention with T-cells from a blood sample will result in the activation and proliferation of specific CTLs in the sample. This activation and proliferation can be assayed for diagnostic purposes. The invention also provides a method for diagnosing...