We demonstrated earlier that replacing the LEDGF/p75 chromatin interaction domain with an alternative DNA-binding protein could retarget integration. Here, we show that transient expression of the chimeric protein using mRNA electroporation efficiently redirects lentiviral vector (LV) integration in wild-...
We demonstrated earlier that lentivector integration can be retargeted by replacing the LEDGF/p75 chromatin interaction-binding domain with an alternative DNA-binding protein, such as CBX1. CBX1 binds di- or trimethylated histone H3K9, that is associated with heterochromatin and intergenic regions, ...